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5ans

216views

What transfection reagent yields high silencing efficiency for siRNA transfection in HepG2 cells?

Compliance WWang9305 2026-09-03 #siRNA #Transfection reagent #HepG2

5ans

165views

Which transfection reagent offers high efficiency and low cytotoxicity for transfecting plasmids into HEK293T cells?

Reagent Selection 黄黄宇569 2026-09-11 #Transfection reagent #HEK293T #Lentiviral packaging

5ans

212views

Does the direct switch of CHO cells from serum-containing to serum-free medium require an adaptation step?

Medical Devices LLisaLab 2026-09-01 #Serum-free medium #CHO cells #Domestication

5ans

256views

Will the antibody expression level of 293 cells drop after replacing serum with serum-free medium?

Storage & Shipping BBuffer9515 2026-09-11 #Serum-free medium #HEK293 #Antibody expression

5ans

242views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

Recombinant Biotinyl...

5ans

181views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

5ans

205views

Can flow cytometry antibodies for surface staining be used for intracellular staining after fixation and permeabilization?

Compliance DDavidScience1321 2026-08-30 #Flow cytometry antibodies #Intracellular staining #Th17

Recombinant Biotinyl...

5ans

264views

Has anyone compared prices for switching the IHC secondary antibody from a universal type to an anti-rabbit/mouse dual-label one?

Supplier & Sourcing RReagentPlus3077 2026-09-01 #Immunohistochemistry #IHC secondary antibody #Double-labeled

5ans

203views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

4ans

240views

For CRISPR knockout in HeLa cells, please recommend Cas9 protein and sgRNA kits.

Storage & Shipping 朱朱娟6512 2026-06-02 #CRISPR-Cas9 #sgRNA #Gene knockout

4ans

194views

Seeking advice on antibody panel design for IL-17A and Foxp3 in Th17/Treg flow cytometry using mouse spleen single cells.

Compliance 陈陈小妹4696 2026-05-07 #Flow cytometry #Th17 #Treg

Recombinant Biotinyl...

4ans

249views

What validation is required for monoclonal antibodies used in ChIP experiments?

Reagent Selection OOscar4174 2026-05-16 #Monoclonal antibody #ChIP #Transcription factor

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