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5ans

212views

Can flow cytometry antibodies for surface staining be used for intracellular staining after fixation and permeabilization?

Compliance DDavidScience1321 2026-08-30 #Flow cytometry antibodies #Intracellular staining #Th17

Recombinant Biotinyl...

5ans

176views

Immunofluorescence tissue sections require Alexa Fluor 594 anti-mouse secondary antibody; which supplier has the fastest availability from stock?

Medical Devices WWendy1455 2026-09-14 #Immunofluorescence #Alexa Fluor 594 #Donkey Anti-Mouse

Recombinant Alexa Fl...

5ans

203views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

5ans

229views

For WB detection of a phosphorylated protein in mouse liver tissue, how should the primary antibody be selected?

Lab Techniques KKevin5578 2026-09-17 #Western blot #Primary antibody #Mouse tissue

5ans

218views

How should the remaining wells of an ELISA kit microplate be stored after stripping the strips? Is the inter-batch variation large?

Compliance DDavid 2026-08-27 #ELISA kit #Microplate #Strip

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

314views

The sample volume of cell culture supernatant is very limited. What is the minimum volume in microliters that can be used with the ELISA kit?

Supplier & Sourcing 余余洋辉 2026-09-07 #ELISA kit #Cell supernatant #Small sample

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

212views

For batch testing of rat TNF-α, how do the lead time and price of a 96T ELISA kit compare?

Storage & Shipping 杨杨娜2860 2026-09-12 #ELISA kit #TNF-α #Rat

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

325views

For human IL-6 ELISA kits used to measure serum samples, are there large differences in recovery rates among different brands?

Compliance VVivi3152 2026-08-31 #ELISA kit #IL-6 #Serum samples

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

237views

Can group buying or tiered pricing be negotiated for bulk purchases of NGS library preparation kits?

Compliance JJackLab4776 2026-08-30 #Procurement price comparison #Tumor panel #Whole exome

5ans

207views

When using the ABI StepOnePlus for mouse gene expression, how should I choose between ROX and dye-based methods?

Reagent Selection 董董飞飞 2026-09-18 #qPCR kit #ROX #ABI StepOnePlus

Recombinant SARS-CoV...

4ans

235views

How to choose Oligo dT resin for in vitro transcribed mRNA vaccine purification?

Lab Techniques 张张磊6040 2026-08-18 #mRNA vaccine #In vitro transcription #Purification resin

4ans

162views

Where can I purchase microfluidic chips for LNP encapsulation of mRNA vaccines through price comparison?

Supplier & Sourcing MMediaing1356 2026-04-26 #mRNA vaccine #LNP encapsulation #Microfluidic chip

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