Tag

#Western Blot

All questions >

3ans

183views

Can domestic alternatives be used for WB primary antibody diluent? How much will the titer drop with repeated use?

Lab Techniques VVivi 2025-09-28 #Antibody Diluent #Western blot #Domestic substitution

0ans

180views

Imported antibodies are several times more expensive than domestic ones. How much profit is actually made?

Medical Devices SSampleBase9086 2026-05-08 #Antibody Procurement #Price difference #Profit margin

0ans

303views

For near-infrared fluorescent dyes used as secondary antibodies in Western blot, how much difference in sensitivity is there between domestic and imported products?

Supplier & Sourcing AAntibodyZone5294 2026-04-15 #Western blot #Near-infrared fluorescence #Secondary antibody

0ans

209views

How many times difference is there between the prices of domestic and imported primary antibodies, and how can I buy small-pack trial sizes?

Storage & Shipping RResearch 2025-11-06 #Western blot #Primary antibody #Domestic substitution

0ans

192views

Which is more suitable for tissue protein extraction: domestic protease inhibitor cocktail or imported aliquoted product?

Supplier & Sourcing NNinaProtein 2026-04-15 #Protease inhibitor #Protein extraction #Western blot

0ans

265views

Where do most people purchase their commonly used WB primary antibodies from which biological reagent company? Is the delivery time stable?

Lab Techniques AAmyBuffer2273 2026-06-24 #Antibody #Bioreagent company #Western blot

Plant PDH E1αElisa K...

3ans

210views

Which brand of ready-to-use Western primary antibody runs the internal reference most stably?

Compliance CCindy1128 2026-07-09 #Western blot #Loading control antibody #Ready-to-use primary antibody

3ans

222views

Which supplier provides Western blot internal reference antibodies with fast delivery and small batch-to-batch variation?

Storage & Shipping 采采购老手 2026-01-27 #Antibody Procurement #Western blot #Loading control antibody

Plant PDH E1αElisa K...

4ans

218views

High background when using Triton X-100 for protein extraction from mouse brain tissue; seeking lysis conditions.

Reagent Selection 阿阿宝小助手2275 2026-05-24 #Triton X-100 #Western blot #Mouse hippocampus

5ans

240views

Will 1% Triton X-100 affect the results when using a BCA kit to measure protein in cell lysates?

Reagent Selection PProteinLab5271 2026-09-24 #BCA protein quantification #Cell lysis buffer #Detergent compatibility

5ans

197views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

5ans

219views

For WB detection of a phosphorylated protein in mouse liver tissue, how should the primary antibody be selected?

Lab Techniques KKevin5578 2026-09-17 #Western blot #Primary antibody #Mouse tissue

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

#Western Blot

Community Rules

Incentives