Compliance - BioReagent Hub

5ans

284views

Most recombinant proteins expressed in E. coli are in inclusion bodies. Are there any mild refolding protocols?

Compliance RResearch9709 2026-09-19 #Recombinant protein #Inclusion bodies #Refolding

5ans

189views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

5ans

281views

When purchasing flow cytometry antibodies, is there a big difference in practice between different brands with the same clone number?

Compliance RResearchZone 2026-09-09 #Flow cytometry antibodies #Brand comparison #CD25

5ans

213views

Can flow cytometry antibodies for surface staining be used for intracellular staining after fixation and permeabilization?

Compliance DDavidScience1321 2026-08-30 #Flow cytometry antibodies #Intracellular staining #Th17

Recombinant Biotinyl...

5ans

214views

For flow cytometry detection of mouse splenocytes, what blocking agent should be used with PE anti-rat secondary antibody to reduce nonspecific binding?

Compliance 何何研发 2026-08-29 #Flow cytometry #PE secondary antibody #Anti-rat

Recombinant Biotinyl...

5ans

183views

Flow cytometry detection of CD molecules in human peripheral blood, direct-labeled primary antibody recommendations for clones and fluorophore combinations.

Compliance TTonyTube518 2026-09-03 #Flow cytometry #Directly labeled primary antibody #CD molecule

Recombinant Biotinyl...

5ans

222views

How should the remaining wells of an ELISA kit microplate be stored after stripping the strips? Is the inter-batch variation large?

Compliance DDavid 2026-08-27 #ELISA kit #Microplate #Strip

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

333views

For human IL-6 ELISA kits used to measure serum samples, are there large differences in recovery rates among different brands?

Compliance VVivi3152 2026-08-31 #ELISA kit #IL-6 #Serum samples

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

247views

Fecal microbial DNA extraction, insufficient lysis leads to low yield. Any recommendations?

Compliance 周周采购 2026-09-20 #Fecal sample #Microbial DNA #Lysis

5ans

227views

For bulk packaging procurement of whole blood DNA extraction kits, which supplier has a faster delivery time?

Compliance MMedia 2026-08-26 #Whole blood DNA #Bulk packaging #Lead time

5ans

171views

Low purity in plant leaf RNA extraction and interference from polysaccharides and polyphenols: what should be done?

Compliance TTony4572 2026-09-19 #RNA extraction #Polysaccharides and polyphenols #Plant sample

5ans

237views

Can group buying or tiered pricing be negotiated for bulk purchases of NGS library preparation kits?

Compliance JJackLab4776 2026-08-30 #Procurement price comparison #Tumor panel #Whole exome

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