Supplier & Sourcing - BioReagent Hub

4ans

296views

Inquire about the prices and lead times of different brands of PMSF. How should bulk procurement for the animal facility be selected?

Supplier & Sourcing PPlateMax 2026-05-26 #Phenylmethylsulfonyl fluoride #Procurement price comparison #Lead time

4ans

213views

What final concentration and incubation time should be used when treating mouse splenocytes with DTT for flow cytometry?

Supplier & Sourcing 李李文博 2026-03-26 #Flow cytometry #DTT #Mouse splenocytes

4ans

196views

For primary cells extracted from porcine tissue, should the penicillin-streptomycin dosage be doubled?

Supplier & Sourcing BBufferLine 2026-08-19 #Penicillin-Streptomycin #Primary extraction #Porcine tissue

4ans

212views

How much does DMSO residue in monoclonal antibody purification samples affect mouse tail vein injection?

Supplier & Sourcing BBuffering7757 2026-06-29 #Dimethyl sulfoxide #Monoclonal antibody purification #Mouse injection

4ans

209views

Can fetal bovine serum still be used for animal cell culture after repeated freeze-thaw cycles?

Supplier & Sourcing GGenePro 2026-09-15 #Fetal Bovine Serum #Repeated freeze-thaw #Cell culture

0ans

185views

What are the general standards for endotoxins and particulate matter in GMP-grade PBS buffer?

Supplier & Sourcing FFlasking4654 2026-09-14 #GMP-grade reagent #PBS buffer #Stem cell preparations

5ans

198views

For intracellular flow cytometry staining of IL-17A and IFN-γ, must the stimulant and blocking agent be from the same brand?

Supplier & Sourcing BBuffer1209 2026-09-05 #Flow cytometry #Intracellular cytokines #Stimulant

5ans

258views

Has anyone compared prices for switching the IHC secondary antibody from a universal type to an anti-rabbit/mouse dual-label one?

Supplier & Sourcing RReagentPlus3077 2026-09-01 #Immunohistochemistry #IHC secondary antibody #Double-labeled

5ans

196views

For Western blotting, when using an HRP-labeled goat anti-rabbit secondary antibody at a 1:5000 dilution, what should I do if the background is still high?

Supplier & Sourcing KKitHub1516 2026-09-15 #Western blot #HRP-conjugated secondary antibody #High background

5ans

305views

The sample volume of cell culture supernatant is very limited. What is the minimum volume in microliters that can be used with the ELISA kit?

Supplier & Sourcing 余余洋辉 2026-09-07 #ELISA kit #Cell supernatant #Small sample

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

237views

High-fidelity enzyme amplification of high-GC templates always results in smearing. How can the system be optimized?

Supplier & Sourcing EEmmaTube4361 2026-09-24 #High-fidelity enzyme #High GC template #System optimization

4ans

151views

Where can I purchase microfluidic chips for LNP encapsulation of mRNA vaccines through price comparison?

Supplier & Sourcing MMediaing1356 2026-04-26 #mRNA vaccine #LNP encapsulation #Microfluidic chip

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