Medical Devices - BioReagent Hub

4ans

225views

What should be added to DMEM when isolating lymphocytes from rat spleen for culture? What is the lead time?

Medical Devices LLily 2026-06-27 #DMEM medium #Primary cell culture #Rat spleen

5ans

180views

Our lab needs to measure SOD and POD activities in over 200 plant leaf samples. Which company's biochemical assay kits are more cost-effective?

Medical Devices LLisa1566 2026-09-05 #Biochemical reagent kit #Antioxidant enzymes #Plant leaves

5ans

214views

Does the direct switch of CHO cells from serum-containing to serum-free medium require an adaptation step?

Medical Devices LLisaLab 2026-09-01 #Serum-free medium #CHO cells #Domestication

5ans

247views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

Recombinant Biotinyl...

5ans

221views

How should a titration experiment be performed after newly purchasing anti-human CD19-PE flow cytometry antibody?

Medical Devices 刘刘小小 2026-09-17 #Flow cytometry antibodies #Titration experiment #CD19

5ans

264views

Consultation on antibody color configuration and usage for flow cytometry CD3/CD4/CD8 in mouse splenocytes

Medical Devices 彭彭明燕 2026-09-04 #Flow cytometry antibodies #Fluorescence color scheme #Mouse splenocytes

Recombinant Biotinyl...

5ans

171views

Immunofluorescence tissue sections require Alexa Fluor 594 anti-mouse secondary antibody; which supplier has the fastest availability from stock?

Medical Devices WWendy1455 2026-09-14 #Immunofluorescence #Alexa Fluor 594 #Donkey Anti-Mouse

Recombinant Alexa Fl...

5ans

161views

How should adapter dimers be optimized when they are too high after微量cfDNA library preparation?

Medical Devices LLisa7850 2026-09-04 #cfDNA #Linker dimer #Micro-library construction

5ans

200views

Can the fragmentation enzyme in the library preparation kit replace sonication shearing?

Medical Devices RReagent8049 2026-09-18 #Metagenomics #Enzymatic fragmentation #Library construction time

5ans

226views

Our lab wants to purchase qPCR master mix in bulk. How should we compare prices between domestic and imported products?

Medical Devices 刘刘超敏 2026-09-07 #SYBR Green #qPCR premix #Bulk purchase

5ans

312views

Low SYBR Green qPCR amplification efficiency: which kit should be used for low-copy templates?

Medical Devices KKevin 2026-09-10 #qPCR kit #Low-copy template #Amplification efficiency

4ans

211views

Is the capillary electrophoresis kit for mRNA vaccine capping efficiency detection in stock?

Medical Devices 陈陈生工 2026-06-23 #mRNA vaccine #Capillary electrophoresis #Quality control kit

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