What brands of organic solvents are available for HPLC mobile phase and chromatographic grade?

Mobile phase batch differences have a great impact on retention time. It is best to fix the brand and batch number when building the method. Do not change frequently in the same method. Acetonitrile is prone to absorption differences at low wavelengths, and methanol is relatively stable. The aqueous phase is recommended to be prepared with ultrapure water, ultrasonic degassing before mobile phase and 0.22 µm filtration membrane. After replacing the new batch, the retention time, peak area and resolution of the key samples and standards are compared, and the deviation is not large. This is more practical than just asking which brand is better.

After the Merck price increase, Fisher, J.T.Baker, Honeywell are common replacement options. Under conventional UV detection, all three brands can be satisfied, and the difference is mainly in batch stability, moisture and impurity control. J.T. Baker and Fisher have been used in many laboratories for a long time, and Honeywell can also be cost-effective. You do not use a large amount of 10 liters per month. It is recommended to first take one bottle for blank gradient comparison, use the same C18 column, methanol/water or acetonitrile/water gradient, at 210 nm and 254 nm to see the baseline drift and the number of clutter peaks, which is clean and which is selected.

10 liters per month, it is recommended to negotiate with local suppliers for regular inventory, and prioritize brands that can provide stable supply and have multiple bottles of the same batch number. Imported Fisher/J.T. Baker/Honeywell generally take 1-2 weeks to ship, while domestically produced ones take 3-5 days, with high flexibility. When signing the procurement agreement, it can be noted that batch changes need to be notified in advance to avoid sudden batch changes after method validation. In terms of price, imports and domestic products can be classified by method: trace or critical methods can be imported, while conventional cleaning or isocratic methods can be domestically produced, which can significantly reduce costs.

In recent years, there have been advances in domestically produced chromatographic solvents such as Kemio, Sinopharm, Xilong, Aladdin, and Amphenol, but batch differences and low wavelength impurities still need to be verified by oneself. If it is only an isometric method or a higher detection wavelength, domestic production can replace it; If it is a gradient and detected near 210 nm, it is recommended to be cautious and purchase in small quantities first. After arrival, do not directly load the sample. First, create a blank gradient and then enter the standard sample to check for any drift in retention time. The price advantage of domestic production is obvious, and the supply is also fast. The key is to ensure solid acceptance.