Can reverse transcriptase be sold as a whole in bulk packaging? How is the validity period calculated

Large packaging or OEM procurement should focus on intra - and inter batch differences, cold chain records, whether samples are provided, and whether small batch customization is acceptable. 100mL pre mixed liquid packaging requires more pollution prevention. It is recommended to operate in a biosafety cabinet or ultra clean bench, and randomly sample for sterile and nuclease/endotoxin testing after packaging. If the manufacturer can provide pre installation services, the priority should be increased.

The shelf life after opening and aliquoting cannot be calculated directly based on the original packaging shelf life. It is recommended to store aliquots at -20°C and perform Ct comparison using the same RNA sample before first use and at 1, 3, and 6 months of storage. If the activity decreases by more than half a Ct or the product yield is significantly reduced, the aliquot storage time should be shortened or the enzyme amount appropriately increased, rather than continuing to use under the original conditions.

Look for domestic original manufacturers with production capacity rather than companies that only act as agents. Inquire directly about custom bulk packaging or OEM, which can usually provide 50,000 U or larger specifications. A monthly volume of over a hundred reactions is sufficient to negotiate packaging specifications with the manufacturer. Clarify the minimum order quantity, shelf life, batch inspection reports, and cold-chain shipping methods. Purchasing by individual unit in small quantities is not recommended.

After the arrival of the large package, it is best to use sterile low suction centrifuge tubes to divide it into single doses on ice or in a cold room to avoid repeated freezing and thawing of the entire bottle. After packaging, it is recommended to store at -20 ℃, mark the date on each tube, and regularly take one tube for activity verification. It is conservative to control the number of repeated freeze-thaw cycles within 3-5 times, and the specific stability data from the manufacturer shall prevail.