The delivery time of the nucleic acid extraction kit is too long, can the laboratory replace it?

Domestic plain replacement of cellular genomic DNA can give priority to finding universal genome extraction kits. Columns or magnetic beads can be used. The key is not in the brand, but whether it is stable in cleavage, protein removal, washing, and elution. Contrast dimensions include OD260/280 and 260/230, Qubit quantification, fragment size, PCR repeatability, post-library insertion fragments, and uniformity. When the monthly usage is large, it is recommended to require the supplier to provide batch samples, MSDS, quality inspection reports, and confirm the adaptation of 96-well plates or magnetic racks to reduce switching consumables. The target unit price can talk about batches, but do not use low prices as the only indicator.

Flattening should not be completely cut at one time. It is recommended to do a three-stage verification: first, use high and low-volume samples of historical cell lines to extract in parallel to compare yield and purity; then run the same set of PCR or targeted amplification to see Ct fluctuations; finally, use the NGS library to look at coverage, GC bias, and connector pollution. If the original import is column type, you can find similar column type domestic products to avoid simultaneous replacement of methods and consumables; if you change magnetic beads, you need to redo the elution volume, amount of magnetic beads, incubation time and magnetic shelf parameters. When the delivery period is unstable, the supplier can prepare the goods in batches and keep at least two batches of comparison.