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All Answers2
L
Leo
28 days ago
实际踩坑多在磁珠、冷链和下游兼容。磁珠残留可用qPCR内标回收或核酸纯度/吸光比辅助判断，同时关注洗脱液离子残留对扩增的影响；得率波动要核对裂解效率、磁珠重悬状态、磁架适配和洗脱温度。运输冷链别只问常温还是冷藏，要确认控温包装、温度指示剂或记录仪，并到货后做小样本性能抽检。建议至少两家做平行验证，同一批盲样分两天跑，看批内重复、批间趋势和仪器报错率，再决定主供和备选。
B
Bio9810
2026-10-02
筛临床核酸提取厂家，先做文件合规和批控：要求提供样本类型适用说明、验证资料、批号追溯、效期管理和运输条件证明，尤其是血清、咽拭子两类基质的数据。不要只看得率，还要看抑制物去除、回收率波动、自动化平台兼容和仪器适配。可要求试用装、装机培训和完整IFU，并在试用时覆盖不同上样体积、洗脱体积和操作人员，记录失败样本与重测情况。采购前让供应商明确异常批处置、投诉响应和批间一致性保障机制。
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