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All Answers2
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CellPro
2026-08-02
口岸监测优先考虑 RT-qPCR/LAMP 兼容性和运输稳定性。选型时建议让供应商提供保存液不含强抑制成分的说明,并关注管体密封、拭子吸附量。可拿两批样品做平行采样:同一拭子在常温放置 24/48/72 小时后提取,比较内参 Ct 漂移和扩增曲线,同时做无模板对照看假阳性。若口岸温度高,优先选常温型但建议做链路验证;若关注生物安全,可评估灭活型,但要看灭活液是否影响后续提取。货期一周内建议锁定稳定批次和区域仓,避免混批。
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Assay1583
2026-10-05
从现场流程看,口岸样本常受高温、日晒和搬运影响,单纯看现货不够。建议把供应商分为常规现货型和稳定批次型,要求提供批次号追溯、包装跌落和密封验证资料。灭活性能不要只问是否灭活,还要问灭活后核酸是否可稳定回收、是否适配自动提取和 LAMP 试剂体系。可设计小批量试用:按实际采样、转运、到实验室时间做链路测试,记录样本外观、渗漏、PCR 抑制和内标结果。若前期出现过提取浓度偏低,可换低吸附拭子或更高回收率保存液。
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