How much does it cost to intraperitoneally inject anti TNF - α monoclonal antibody into mice for an experiment?

Domestic alternatives can be tried, but the focus is not on the price, but on whether the clone number, neutralization activity, endotoxin and dosage form are suitable for in vivo injection. Many common ELISA/flow antibodies contain sodium azide or high endotoxin and cannot be injected directly intraperitoneally. It is recommended to prioritize products labeled “In vivo functional grade” or “Ultra-LEAF” and request endotoxin, sterility, preservative-free and purity reports. Buy the minimal package first and perform a pre-test in parallel with the commonly used clones in the literature to see if the pathological scores, body weight changes, and cytokine indicators are consistent. Amortize the single cost of 500 µg or 1 mg specifications to the number of animals per group when requesting a quote. Domestic suppliers can be used as a long-term procurement option if the delivery period is short and they can provide the same batch of stock.

Suggest converting by quality first, not by quantity. If the mouse weighs 20 g, 10 mg/kg is 0.2 mg/mouse, which is 200 µ g/mouse. The 100 µ g specification is not enough for one injection, at least 500 µ g or 1 mg should be purchased. When estimating the cost, multiply the unit price per mg by 0.2 mg per unit, rather than looking at the price per unit. When inquiring, ask the supplier to provide a unified quote of "price per mg+minimum specification+whether freight and cold chain are included". The price per mg of research grade anti TNF - α monoclonal antibodies is usually higher than that of therapeutic analogues, and the channel differences are also significant; Domestic functional grade products are often cheaper than imported ones, but it is necessary to clarify whether there is neutralizing activity data. The delivery time for domestic spot goods is generally 3-7 days, for imports it takes 2-4 weeks, and the entire process is cold chain at 2-8 ° C. After arrival, package according to single usage to avoid repeated freezing and thawing. If the model dose literature has upper and lower limits, reserve an additional 20% -30% loss when stocking.

Suggest creating a comparison table: supplier, clone number, specifications, unit price per mg, delivery time, storage conditions, presence of preservatives, and batch activity/endotoxin reports. Do not choose ordinary products containing sodium azide or multi antibody serum for in vivo experiments; It is best to use PBS or low endotoxin buffer for abdominal injection of antibodies, and confirm the protein concentration and purity. After the cold chain arrives, check the temperature record first. Do not rush to package before opening the lid, and confirm whether there is a sterile packaging environment. When packaging, follow the dosage of each experimental group, for example, use 0.2 mg/piece at a time, and prepare 2-3 mg/tube for 10 pieces. After packaging, store at -20 or -80 to avoid repeated freezing and thawing; Repeated freeze-thaw cycles may cause aggregation, affecting the reproducibility of animal experiments. It is recommended to cover at least the same batch of pre experiments and formal experiments in terms of procurement quantity, in order to avoid changing batches midway.