Can the plasmid extraction kit on the official website of Biyun Tian remove endotoxins?

Ordinary plasmid trays without the words' endotoxin free 'in their names generally cannot guarantee endotoxin removal, and the lack of EU values in the instructions is equivalent to no verifiable indicators. First, download the instruction manual according to the item number and search for "endotoxin residue", "endotoxin level", or whether there are endotoxin clearance steps. HEK293T is sensitive to transfection grade plasmids, and it is recommended to prioritize using small extraction kits that clearly indicate the presence of endotoxins; If the customer service only asks to read the instruction manual and asks the technical support for the "endotoxin testing method and typical range for this batch", caution should be taken when using it for critical transfection.

If you want to buy a genuine endotoxin removal small extraction box for less than 20 yuan per transaction, your options may be limited, and you need to carefully calculate the cost per transaction: some products that claim to remove endotoxins may have an additional cleaning or endotoxin removal column, and the cost per transaction may be higher than ordinary small extraction boxes. If the budget is tight, the total single cost of the combination of "ordinary small lifting box+endotoxin removal centrifuge column/reagent" can be compared. Do not directly transfect ordinary Xiaoti with 293T just for the sake of cheapness. Before purchasing, ask customer service to provide the single processing capacity and suggested washing volume, and verify in small batches upon arrival.

5 ml overnight high-copy bacterial solution, the conventional small extraction rate is usually a few μg to more than twenty μg, but the carrier type, strain, and shaking time have a great influence, and it is not possible to only look at the column load. It is recommended that the Nanodrop concentration and OD260/280 should be tested after completion. About 1.8-2.0 indicates that the nucleic acid purity is acceptable, but the normal ratio does not mean that the endotoxin is low. It is best to use the lysate reagent to test the endotoxin, or transfer 293T to observe the cell state. Endotoxin removal kits usually lose a part of the yield and give priority to ensuring the quality of the transfection.