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4ans

210views

Immunofluorescence of fish tissue sections, permeabilization concentration of Triton X-100 and procurement issues.

Lab Techniques PPCRMax5123 2026-09-19 #Triton X-100 #Immunofluorescence #Zebrafish

4ans

177views

How much activity is lost when bulk trypsin is aliquoted and subjected to repeated freeze-thaw cycles?

Lab Techniques 老老王小陈5167 2026-03-09 #Trypsin #Aliquot and store #Repeated freeze-thaw

4ans

216views

Can fetal bovine serum still be used for animal cell culture after repeated freeze-thaw cycles?

Supplier & Sourcing GGenePro 2026-09-15 #Fetal Bovine Serum #Repeated freeze-thaw #Cell culture

5ans

339views

After the recombinant cytokine lyophilized powder is dissolved, repeated freeze-thaw cycles cause a severe drop in activity. What should be done?

Storage & Shipping 林林小小 2026-08-28 #Recombinant cytokines #Lyophilized powder #Activity

5ans

258views

After thawing fetal bovine serum, if white flocculent precipitate appears, can it still be used?

Lab Techniques 孙孙鹏 2026-09-09 #Fetal Bovine Serum #Primary culture #Serum precipitate

4ans

186views

How should the dosage of anti-human CD3 monoclonal antibody for flow cytometric detection of human T cell surface be titrated?

Reagent Selection AAntibodyZone1727 2026-05-03 #Monoclonal antibody #Flow cytometry #CD3

5ans

207views

When stimulating PBMC with recombinant human cytokines, how should they be aliquoted and stored to prevent degradation?

Storage & Shipping WWang7665 2026-09-09 #Recombinant protein #Cytokine #Aliquot and store

4ans

256views

Can reverse transcriptase be sold as a whole in bulk packaging? How is the validity period calculated

Reagent Selection 研研发老师5514 2026-07-12 #Reverse transcriptase #Bulk packaging #Aliquoting

4ans

184views

Will repeated freezing and thawing of cell cryopreservation solution after packaging affect its effectiveness? Commonly used large packaging in laboratories

Lab Techniques TTom7693 2026-08-29 #Aliquot and store #Repeated freeze-thaw #Cell cryopreservation solution

4ans

319views

After preparing HRP and storing it in a refrigerator at 4 degrees Celsius, how long can the activity generally remain stable without decreasing?

Lab Techniques SSample5751 2026-02-24 #HRP storage #Activity stability #Western blot

4ans

218views

How to dilute and store SYBR Green I mother liquor? Repeated freeze-thaw cycles will result in failure

Reagent Selection 徐徐刚413 2026-04-14 #Storage conditions #Aliquoting #SYBR Green I

4ans

233views

How many times can dNTPs be repeatedly frozen and thawed when stored at -20 ℃?

Supplier & Sourcing EEmmaTube 2026-04-11 #dNTPs #Aliquoting #Freeze-thaw

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