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4ans

169views

How much activity is lost when bulk trypsin is aliquoted and subjected to repeated freeze-thaw cycles?

Lab Techniques 老老王小陈5167 2026-03-09 #Trypsin #Aliquot and store #Repeated freeze-thaw

4ans

207views

Can fetal bovine serum still be used for animal cell culture after repeated freeze-thaw cycles?

Supplier & Sourcing GGenePro 2026-09-15 #Fetal Bovine Serum #Repeated freeze-thaw #Cell culture

5ans

330views

After the recombinant cytokine lyophilized powder is dissolved, repeated freeze-thaw cycles cause a severe drop in activity. What should be done?

Storage & Shipping 林林小小 2026-08-28 #Recombinant cytokines #Lyophilized powder #Activity

5ans

253views

After thawing fetal bovine serum, if white flocculent precipitate appears, can it still be used?

Lab Techniques 孙孙鹏 2026-09-09 #Fetal Bovine Serum #Primary culture #Serum precipitate

4ans

179views

How should the dosage of anti-human CD3 monoclonal antibody for flow cytometric detection of human T cell surface be titrated?

Reagent Selection AAntibodyZone1727 2026-05-03 #Monoclonal antibody #Flow cytometry #CD3

5ans

202views

When stimulating PBMC with recombinant human cytokines, how should they be aliquoted and stored to prevent degradation?

Storage & Shipping WWang7665 2026-09-09 #Recombinant protein #Cytokine #Aliquot and store

4ans

181views

Will repeated freezing and thawing of cell cryopreservation solution after packaging affect its effectiveness? Commonly used large packaging in laboratories

Lab Techniques TTom7693 2026-08-29 #Aliquot and store #Repeated freeze-thaw #Cell cryopreservation solution

4ans

253views

How long can restriction endonucleases be stored stably at -20 ℃ after being packaged in large packages?

Supplier & Sourcing WWendySample2407 2026-09-01 #Restriction endonuclease #HindIII #Aliquot and store

4ans

280views

Can ordinary EP tubes be used for aliquoting and storing bioreagent AB?

Storage & Shipping 实实验小哥 2026-05-14 #Biological reagent AB #Aliquot and store #Low-binding consumables

3ans

209views

What should we do if the serum thawing time is too long and we urgently need to prepare the complete culture medium?

Lab Techniques LLeo7100 2026-08-25 #Fetal Bovine Serum #Thawing time #Aliquot and store

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