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Literature喜报|翌圣反转录产品连发两篇高分文章

📂 qPCR系列 Type ID 5004

👁 12,273 views 🗓 2026-10-13 Source ID：2384

近日，陶亮博士在国际学术期刊Cell Death & Differentiation（IF 10.717）发表研究论文，对NASH/NAFLD治疗药物及其作用靶点进行了总结，为NAFLD 新药研发提供参考。

Source: Yeasen Biotech ｜ Original: https://www.yeasen.com/solutiondetail/2384 ↗

非酒精性脂肪肝病(NAFLD)是最常见的慢性肝病之一，全球发病率高达25%，是全世界公共健康的一大威胁。其中非酒精性脂肪肝炎(NASH) 可导致肝硬化和肝癌的发生，是终末期肝病和肝移植的主要原因之一。NASH 发病机理复杂，目前尚无有效药物。因此，巨大的市场需求使NASH 新药研发成为全球制药公司追逐的热点。

近日，陶亮博士在国际学术期刊Cell Death & Differentiation（IF 10.717）发表题为：RIP1 kinase activity promotes steatohepatitis through mediating celldeath and inflflammation in macrophages的研究论文（点此查看原文），对NASH/NAFLD治疗药物及其作用靶点进行了总结，为NAFLD 新药研发提供参考。

值得一提的是，该团队选择了翌圣反转录试剂盒（Cat NO.11119ES）用于一链cDNA的合成。Anti-RIPK1/ RIP1 phospho (Ser166) anti-body (YJY-1-5) was from Arigobio. Lipopolysaccharides(LPS) was from Sigma-Aldrich. Nigericin (481990) was from Merck-Millipore (Germany). Penicillin–streptomycin, liquid (15140122) were from GIBCO (America).Hifair^®II 1st Strand cDNA Synthesis Kit (11119ES60) was from Yeasen(Shanghai, China).

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此外，上海药物研究所黄永焯团队近期在Journal of controlled release（IF 7.727）发表的题为Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brainmetastasis via macrophage-mediated innate immunity的研究论文也选择了翌圣的反转录预混液（Cat NO.11123ES）和qPCR预混液（Cat NO.11202ES）等系列产品。（点此查看原文）

The qPCR SYBR^® Green Master Mix, cDNA synthesis SuperMix kit, and TRIeasy™ Total RNA Extraction Reagent were from Yeasen Biotech Co., Ltd. (Shanghai, China).All primers for q-PCR were synthesized by Generay Biotech Co., Ltd. (Shanghai, China).下面给大家介绍一下翌圣的反转录相关产品。

翌圣反转录产品选择指南

反转录产品性能

1、出色的反转录效率，适合不同 GC 含量、不同表达丰度的基因

图1.Hifair^® Ⅲ 预混液(Cat NO.11141ES)相对市面同类产品具有更高的逆转录效率。以300ng 293T细胞的总RNA为模板, 使用11141ES、T品牌合成cDNA。取1μL cDNA为模板, 使用Hieff UNICON^® Power qPCR 预混液 (Cat NO.11195ES)扩增20个不同GC含量（25-65%）、不同表达丰度的基因。

2、线性检测范围广，Total RNA 10pg-5μg

图2.Hifair^® Ⅲ SuperMix预混液（Cat NO.11141ES）在宽广的模板范围内均可高效合成cDNA。以10pg-5μg的293T细胞的总RNA为模板，使用11141ES合成cDNA。取1μL cDNA为模板，使用Hieff UNICON^®Power qPCR 预混液 (Cat NO.11195ES)扩增PTTG1基因。翌圣反转定量产品还荣登过这些高分杂志（部分）

[1] Xiao-Yi Lu, Xiong-Jie Shi, Ao Hu, Ju-Qiong Wang, Yi Ding, Wei Jiang, Ming Sun, Xiaolu Zhao, Jie Luo, Wei Qi, Bao-Liang Song. Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis. Nature.（IF 43）[2] Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.（IF31.398） [3] Han X., et al., Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell. 2018 Feb 22;172(5):1091-1107.e17. （IF30.410）[4] Wang X, Ni L, Wan S, et al. Febrile temperature critically controls the differentiation and pathogenicity of T helper 17 cells[J]. Immunity, 2020, 52(2): 328-341. e5. （IF21.522）[5] Han F , Liu X , Chen C , et al. Hypercholesterolemia risk-associated GPR146 is an orphan G-protein coupled receptor that regulates blood cholesterol levels in humans and mice[J]. Cell Research.（IF 20.5）

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Source: Yeasen Biotech

Original URL: https://www.yeasen.com/solutiondetail/2384

Source ID: 2384

Content is compiled from Yeasen Biotech public materials; all rights belong to the original author.

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