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Product data sheet PD-15214ES
BsrDI (10 U/μL) BsrDI (10 U/μL)
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BsrDI (10 U/μL)

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分子克隆Golden Gate组装
Product no.15214ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15214ES25 现货 $408.00 促$425.00
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Cat.No Spec Price Status
15214ES25 250 U $408.00促$425.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15214ES
Specification250 U
Origin翌圣生物科技(上海)股份有限公司
技术平台 分子克隆
应用方法 Golden Gate组装用于DNA片段定向组装
适用物种种属 嗜热脂肪芽孢杆菌
生物特性参数 试剂类型限制性内切酶 应用Golden Gate组装 来源物种Bacillus stearothermophilus D70 酶类型Type IIS型限制酶
技术参数 浓度10 U/μL 储存条件-25~-15℃保存,有效期2年 识别位点5'-GCAATGNN↓-3' 3'-CGTTAC↑NN-5' 反应条件1×BsrDI Buffer;37℃孵育 失活条件80℃温育20 min 同裂酶Bse3DI BseMI 甲基化修饰影响EcoBI:剪切受阻 酶活定义单位活性单位(U)是指在50 μL反应体系中,37℃ 1 h内完全酶切1 µg λDNA所需的酶量
实验类型 限制性酶切用于在特定序列处切割DNA

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