Agarose琼脂糖
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Q：为什么跑出的条带弥散？
A：可能凝胶时间过短，胶不均匀。1%的琼脂糖最少凝胶时间为 30min。Q2：高分辨琼脂糖能够分开多大的条带？
A：高分辨率琼脂糖和 3:1 琼脂糖的分辨率均为 2%左右（即片段 30bp 的话，则能分开 30*0.02；100bp 片段则 100*0.02。）
Q：低熔点琼脂可以用来做脉冲场凝胶电泳吗？
A：不可以，脉冲场凝胶电泳要求是电渗要很低，强度要很高。
Q：低熔点琼脂糖是不是无菌无核酸酶的？其他类型的琼脂糖呢？
A：无菌无核酸酶，低熔点琼脂糖常用来做细胞空斑实验，细胞培养可放心使用。其他类型的琼脂糖也是无菌的，无核酸酶的。
Q：琼脂糖会比较软，这个是什么原因？
A：软硬和凝胶强度有关，琼脂糖都是琼脂提取物，强度高低都是会有一定差异的，不可能每一批都相同。琼脂糖的强度指标是大于等于 1200，只要在这个范围以上就是合格的。
已发表文献
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