Recombinant Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) ,hFc Tag

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HEK293表达系统

Product no.94694ES Sizes3 options SourceYeasen · Official

Cat. No. 94694ES25

现货 ¥2,285.00

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94694ES25 25 μg ¥2,285.00 现货 Enquire

94694ES60 100 μg ¥6,885.00 现货 Enquire

94694ES76 500 μg ¥24,115.00 现货 Enquire

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Specifications

Brand翌圣生物 Yeasen

Cat.No94694ES

Purity> 95% as determined by SDS-PAGE and HPLC.

Specification100 μg

Origin翌圣生物科技(上海)股份有限公司

复溶方法 开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。

内毒素 < 1.0 EU per μg by the LAL method.

种属 Mouse

产品别称 PDCD1 SLEB2 CD279

制剂 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

标签 C-hFc

纯度 > 95% as determined by SDS-PAGE and HPLC.

Accession NP_032824

生物活性 【SPR Data】 Mouse PD-L1 His Tag captured on CM5 Chip via anti-his antibody can bind Biotinylated Mouse PD-1 hFc Tag with an affinity constant of 3.35 μM as determined in SPR assay .

表达序列 Leu25-Gln167

表达系统 HEK293

分子量 The protein has a predicted MW of 42.9 kDa. Due to glycosylation the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

外观 Lyophilized Powder

技术平台 HEK293表达系统

适用物种种属小鼠

检测方法 SDS-PAGE HPLC 鲎试剂法（LAL） 表面等离子共振（SPR）

生物特性参数 蛋白类型重组生物素化蛋白 种属来源小鼠 生物活性描述SPR数据：Mouse PD-L1 His Tag捕获于CM5芯片上，通过抗his抗体可与Biotinylated Mouse PD-1 hFc Tag结合，亲和常数为3.35 μM（SPR测定） 产品别称PDCD1 SLEB2 CD279

技术参数 内毒素水平< 1.0 EU/μg (LAL法) 分子量预测42.9 kDa；糖基化后60-70 kDa 纯度> 95% (SDS-PAGE和HPLC) 制剂形式冻干粉，含0.22μm过滤的PBS (pH7.4) 和8%海藻糖 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月 活性SPR亲和常数 3.35 μM（与Mouse PD-L1结合） 表达序列Leu25-Gln167 复溶方法开盖前离心，用蒸馏水复溶至浓度>100 μg/mL，分装避免反复冻融 Accession号NP_032824 表达系统HEK293 标签C-hFc 外观冻干粉