品牌翌圣生物 Yeasen
货号 / 型号94512ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
适用物种种属
人
检测方法
SDS-PAGE
LAL法
ELISA
生物特性参数
蛋白类型重组人SOST/Sclerostin蛋白
种属来源人
生物活性描述Immobilized Anti-SOST Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SOST
His Tag with the EC50 of 3.9ng/ml determined by ELISA.
应用ELISA检测
产品别称VBCH
sclerosteosis
SOST
sclerostin
DAND6
技术参数
纯度> 95% as determined by SDS-PAGE.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 24.2 kDa. Due to glycosylation
the protein migrates to 28-40 kDa based on SDS-PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
活性EC50 of 3.9 ng/ml determined by ELISA for Biotinylated Human SOST
His Tag.
表达系统HEK293
标签N-His-Avi
外观Lyophilized Powder
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
表达序列Gln24-Tyr213
AccessionQ9BQB4-1
实验类型
SDS-PAGE纯度检测
LAL法内毒素水平检测
ELISA生物活性检测