Recombinant Human RNF43 Protein,hFc Tag

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HEK293表达系统抗体药物靶点研究

Product no.94500ES Sizes3 options SourceYeasen · Official

Cat. No. 94500ES60

现货 ¥3,585.00

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94500ES60 100 μg ¥3,585.00 现货 Enquire

94500ES76 500 μg ¥12,355.00 现货 Enquire

94500ES25 25 μg ¥985.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94500ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究作为抗体药物靶点蛋白用于药物筛选和研究

适用物种种属人

检测方法 SDS-PAGE HPLC LAL法 ELISA

生物特性参数 蛋白类型RING-type E3 ubiquitin transferase RNF43 / E3泛素连接酶 种属来源Human（人） 生物活性描述ELISA实验显示：固定Human R-Spondin 3 His Tag（1μg/ml）时，Human RNF43 hFc Tag的EC50为25.2ng/ml；固定Human R-spondin 1 His Tag（5μg/ml）时，Human RNF43 hFc Tag的EC50为0.4μg/ml。 应用重组蛋白/靶点蛋白/抗体药物靶点蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 45.8 kDa; migrates to 55-60 kDa due to glycosylation by SDS-PAGE. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose added as protectant before lyophilization. 活性ELISA EC50 = 25.2 ng/ml against Human R-Spondin 3; EC50 = 0.4 μg/ml against Human R-spondin 1. 储存条件-25 ~ -15℃保存，收到货后有效期1年。复溶后，无菌条件下-85 ~ -65℃保存，3个月有效期。 表达序列Gly24-Tyr197 标签C-hFc 表达系统HEK293 AccessionQ68DV7-1 外观Lyophilized Powder 复溶方法开盖前先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。