品牌翌圣生物 Yeasen
货号 / 型号94282ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点研究靶点蛋白
适用物种种属
人
检测方法
ELISA
SDS-PAGE
LAL
生物特性参数
蛋白类型重组蛋白
种属来源人
生物活性描述ELISA Data: Immobilized Human LRP-6
mFc Tag at 5μg/ml (100μl/Well) on plate. Dose response curve for Biotinylated Human SOST
His Tag with the EC50 of 0.87μg/ml determined by ELISA.
技术参数
纯度>90% as determined by SDS-PAGE.
内毒素水平<1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 95.3 kDa. Due to glycosylation
the protein migrates to 110-115 kDa based on SDS-PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性Immobilized Human LRP-6
mFc Tag at 5μg/ml (100μl/Well) on plate. Dose response curve for Biotinylated Human SOST
His Tag with the EC50 of 0.87μg/ml determined by ELISA.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达系统HEK293
标签C-mFc
外观Lyophilized Powder
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
AccessionO75581-1
表达序列Ala20-Pro630
实验类型
ELISA生物活性检测
SDS-PAGE纯度检测
LAL内毒素检测