Recombinant Human Beta Klotho Protein, His Tag

Scan for product

FGF信号通路研究

Product no.94201ES Sizes4 options SourceYeasen · Official

Cat. No. 94201ES60

现货 ¥4,445.00

Qty 1

Enquire now

Enquire / bulk Compare

Specifications & Enquiry

Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.

Cat.No Spec Price Status

94201ES60 100 μg ¥4,445.00 现货 Enquire

94201ES76 500 μg ¥14,855.00 现货 Enquire

94201ES80 1 mg ¥29,715.00 现货 Enquire

94201ES25 25 μg ¥1,195.00 现货 Enquire

Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94201ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

应用方法 FGF信号通路研究研究FGF15/19和FGF21信号传导

适用物种种属人

检测方法 酶联免疫吸附试验 表面等离子共振 聚丙烯酰胺凝胶电泳 高效液相色谱

生物特性参数 蛋白类型重组蛋白 种属来源人 (Human) 生物活性描述【ELISA Data】Immobilized Human Beta Klotho at 2μg/ml (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody1 hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. Immobilized Human FGF21 mFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human Beta Klotho His Tag with the EC50 of 1.48μg/ml determined by ELISA. 【SPR Data】Human Beta Klotho His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21 mFc Tag with an affinity constant of 0.54nM as determined in SPR assay. 应用FGF信号通路研究

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Approximately 111.6 kDa. Due to glycosylation the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性高活性 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达序列Met30-Thr983 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 蛋白编号Q86Z14 表达系统HEK293 标签C-His 外观Lyophilized Powder 产品别称betaKlotho beta-klotho BKL KLB klotho beta like Klotho beta β-klotho klotho β like Klotho β