品牌翌圣生物 Yeasen
货号 / 型号94049ES
规格100 μg / 500 μg / 1 mg
产地翌圣生物科技(上海)股份有限公司
应用方法
新冠相关研究S蛋白在诱导中和抗体和T细胞反应及保护性免疫中发挥关键作用
适用物种种属
SARS-CoV-2
检测方法
ELISA
Tris-Bis PAGE
HPLC
LAL
生物特性参数
蛋白类型重组Spike S三聚体蛋白
种属来源SARS-COV-2
生物活性描述ELISA Data: Immobilized SARS-COV-2 Spike S Trimer
His Tag at 1μg/ml (100μl/Well). Dose response curve for Human ACE2
hFc Tag with the EC50 of 42.2ng/ml determined by ELISA.
应用S蛋白在诱导中和抗体和T细胞反应及保护性免疫中发挥关键作用
技术参数
纯度> 95% as determined by Tris-Bis PAGE and HPLC
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 136.70 kDa. Due to glycosylation
the protein migrates to 140-180 kDa based on Tris-Bis PAGE result.
制剂形式Supplied as 0.22μm filtered solution in 10mM PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
储存条件Store at -25~-15℃ for 1 year. After reconstitution: 2~8°C for 1 month
-25~-15°C for 3 months. Aliquot to avoid repeated freeze-thaw cycles.
表达系统HEK293 Cells
标签His tag and Avi tag at the C-terminal
外观冻干粉
复溶方法Centrifuge tubes before opening. Reconstitute to concentration more than 100 μg/mL (usually 1 mg/mL). Dissolve in distilled water.
表达序列Val16-Glu1188
Accession号P0DTC2
活性ELISA EC50 = 42.2 ng/ml against Human ACE2
实验类型
ELISA检测蛋白与人ACE2的结合活性
