品牌翌圣生物 Yeasen
货号 / 型号94047ES
纯度> 95% as determined by SDS-PAGE and HPLC
规格25 μg
产地翌圣生物科技(上海)股份有限公司
技术平台
Expi293
应用方法
病毒学研究用于SARS-CoV-2 Spike蛋白与ACE2受体结合研究
适用物种种属
SARS-CoV-2
检测方法
SDS-PAGE
HPLC
ELISA
LAL
生物特性参数
蛋白类型重组蛋白
种属来源SARS-CoV-2
生物活性描述ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron BA.4/BA.5/BA.5.1.3/BA.5.2)
His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 39.7ng/ml determined by ELISA.
应用用于SARS-CoV-2 Spike蛋白与ACE2受体结合研究
样本类型
重组蛋白
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 26.05 kDa. Due to glycosylation
the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
活性EC50 of 39.7ng/ml determined by ELISA
表达系统Expi293
标签His tag at the C-terminal
表达区间Arg319-Phe541(G339D
S371F
S373P
S375F
T376A
D405N
R408S
K417N
N440K
L452R
S477N
T478K
E484A
F486V
Q498R
N501Y
Y505H)
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
AccessionQHD43416.1
UniProtP0DTC2
产品别称S protein RBD; S glycoprotein RBD; Spike protein RBD
外观冻干粉
实验类型
SDS-PAGE用于纯度检测
HPLC用于纯度检测
ELISA用于结合活性检测
LAL用于内毒素水平检测
