DAPI Solution DAPI染液(5 mg/mL)
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Q: DAPI是一种好的活细胞核标记探针吗?
A: DAPI被认为是一种半渗透性/无渗透性的细胞核染料。对细胞核的染色性能好坏依赖于细胞系类型。某些细胞系可用DAPI染色,某些完全不可以,而某些标记结果不一致。替代的话,建议用Hoechst 33342或Hoechst 33258,具有同DAPI一样的波长和核酸结合模式,但是有非常好的细胞膜渗透性。
Q.DAPI和Hoechst染料相当类似,如何二选一?
A:DAPI是非常通用的蓝色荧光染料,用于细胞核复染。对固定和透化的细胞/组织,具有非常明亮的细胞核标记。然而,此染料被认为是半渗透至不渗透的染料,用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料,具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像,效果就如同DAPI用于固定细胞染色。
已发表文献
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