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Q:如何做到同时染色溶酶体和细胞核的?
A:建议用 Hoechst 33258/ Hoechst 33342 染色细胞核,这个染料可以直接染活细胞不需要固定操作。
Q.DAPI和Hoechst染料相当类似,如何二选一?
A:DAPI是非常通用的蓝色荧光染料,用于细胞核复染。对固定和透化的细胞/组织,具有非常明亮的细胞核标记。然而,此染料被认为是半渗透至不渗透的染料,用于活细胞染色的结合不一致。Hoechst 33342是一种细胞膜渗透性的染料,具有同DAPI的结合机制和荧光特征。非常适合用于活细胞成像,效果就如同DAPI用于固定细胞染色。
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