Anti-DYKDDDDK (Flag) Affinity Gel (Secretory or Membrane Protein) Anti-Flag亲和纯化凝胶(分泌蛋白或膜蛋白）

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亲和层析分泌蛋白或膜蛋白纯化生物活性检测结构解析

Product no.20585ES Sizes5 options Documents1 files SourceYeasen · Official

Cat. No. 20585ES03

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Cat.No Spec Price Status

20585ES03 1 mL $2,018.00促~~$2,325.00~~ 现货 Enquire

20585ES08 5 mL $5,818.00促~~$7,425.00~~ 现货 Enquire

20585ES25 25 mL $28,818.00促~~$32,725.00~~ 现货 Enquire

20585ES60 100 mL $92,818.00促~~$116,025.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20585ES

Specification高特异性捕获：高质量的鼠源IgG2b单克隆抗体（克隆号：3F5）与4%高度交联的琼脂糖凝胶共价偶联，精准识别并结合带有Flag标签的跨膜蛋白，显著减少杂蛋白的非特异性结合，确保纯化后的跨膜蛋白具有高纯度。高载量：即使跨膜蛋白表达量较低，其高

Origin翌圣生物科技(上海)股份有限公司

技术平台 亲和层析

应用方法 分泌蛋白或膜蛋白纯化用于带有Flag标签的分泌蛋白或膜蛋白的纯化 生物活性检测适用于后续生物活性检测 结构解析适用于后续结构解析

生物特性参数 蛋白类型单克隆抗体 表达/来源系统小鼠应用分泌蛋白或膜蛋白纯化 免疫沉淀（IP）

技术参数 制剂配方TBS，50%甘油，pH7.4，含有0.02%（w/v）叠氮钠 储存条件-25~-15℃保存，有效期1年。禁止在不含甘油的溶液中冻存！ 蛋白结合量≥1.1 mg蛋白/mL凝胶 克隆号3F5 亚型Mouse IgG2b 基质4%高度交联的琼脂糖凝胶

实验类型 免疫沉淀用于Flag标签融合蛋白的免疫沉淀 亲和纯化用于Flag标签融合蛋白的亲和纯化

FAQ

Q：假设蛋白要保持活性，用哪些物质洗脱？

A：可以使用非变性洗脱方式。

Q：该产品和进口厂家的比怎么样？

A：Yeasen 20585 蛋白结合量几乎是进口S*品牌的 2 倍。

Q：用 flag 多肽进行洗脱，但是一直洗脱不下来？

A：介质的载量本身就不高，洗脱浓度有可能较低，有可能因浓度较低而看不到明显条带。建议下一步增加洗脱强度。

Documents & Citations

蛋白纯化产品目录 Open document

Published citations

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[2] Han L, Zhang F, Liu Y, et al. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection. Clin Transl Med. 2022;12(6):e850. doi:10.1002/ctm2.850(IF:11.492)

[3] Chen X, Duan Y, Qiao F, et al. A secreted fungal effector suppresses rice immunity through host histone hypoacetylation [published online ahead of print, 2022 May 20]. New Phytol. 2022;10.1111/nph.18265. doi:10.1111/nph.18265(IF:10.152)

[4] Chen Y, Yang L, Lu Y, et al. Up-regulation of NMRK2 mediated by TFE3 fusions is the key for energy metabolism adaption of Xp11.2 translocation renal cell carcinoma. Cancer Lett. 2022;538:215689. doi:10.1016/j.canlet.2022.215689(IF:8.679)

[5] Chen X, Li X, Li P, et al. Comprehensive identification of lysine 2-hydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2-hydroxyisobutyrylation in fungal virulence. J Integr Plant Biol. 2021;63(2):409-425. doi:10.1111/jipb.13066(IF:7.061)

[6] Tang C, Zhou Y, Sun W, et al. Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway. Cancers (Basel). 2022;14(9):2338. Published 2022 May 9. doi:10.3390/cancers14092338(IF:6.639)

[7] Zheng G, Jiang C, Li Y, et al. TMEM43-S358L mutation enhances NF-κB-TGFβ signal cascade in arrhythmogenic right ventricular dysplasia/cardiomyopathy. Protein Cell. 2019;10(2):104-119. doi:10.1007/s13238-018-0563-2(IF:6.228)

[8] Chen X, Tang J, Pei Z, et al. The 'pears and lemons' protein UvPal1 regulates development and virulence of Ustilaginoidea virens. Environ Microbiol. 2020;22(12):5414-5432. doi:10.1111/1462-2920.15284(IF:4.933)