Nuclear and Cytoplasmic Protein Extraction Kit 细胞核/细胞浆蛋白抽提试剂盒
勾选需要的规格可一次提交；也可以逐个询价。货号会一并带入询价单。
Q：组织提取浆蛋白，组织量与提取试剂的比例如何？
A：将组织切成非常细小的碎片。根据后续使用量按照20:1的比例混合试剂A和B，并加入PMSF至最终浓度为1 mM，混匀即可配制成组织匀浆液。按照每60 mg组织加入200 µL组织匀浆液的比例混合组织和组织匀浆液（对于不超过60mg的组织样品，仅需加入100微升组织匀浆液），并在玻璃匀浆器内充分匀浆。
接下来请参照【细胞样品蛋白抽提】的步骤2）开始操作，即把沉淀当做已经离心收集好的细胞沉淀操作。（特别说明：对于起始量为60mg的组织样品，后续直接按照步骤2-7操作即可；对于起始量不超过60mg的组织样品，后续步骤中的溶液的用量须减半使用)。
对于组织样品，如果每个样品的重量不超过60mg，本试剂盒可以抽提50个样品，如果每个样品为60mg，本试剂盒可以抽提25个样品。
Q：该产品是否可以用于冻存组织？
A：对于组织样品，仅适合于新鲜组织，对冻存过的组织抽提效果很差。
Q：使用cocktail代替PMSF可以吗？
A：可以。
Q：这个试剂盒可以用来提取真菌或植物的细胞核膜蛋白吗？
A：本产品不适用于真菌及植物样品的。
Q：细胞沉淀收集后，如果不立马提蛋白，是需要放在-20还是-80保存吗？
A：建议提取蛋白后再保存。样本需要新鲜细胞的，不适合冻存的。
Q：该产品按说明书中所述，核在裂解之前，即使吸干净胞浆液体，会不会胞浆残留在核里面？
A：可以用预冷pbs洗一下，减少浆蛋白污染。
已发表文献
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