Tick the specs you need to submit them together; or enquire one by one. Cat. numbers are carried into the RFQ.
Q:组织提取浆蛋白,组织量与提取试剂的比例如何?
A:将组织切成非常细小的碎片。根据后续使用量按照20:1的比例混合试剂A和B,并加入PMSF至最终浓度为1 mM,混匀即可配制成组织匀浆液。按照每60 mg组织加入200 µL组织匀浆液的比例混合组织和组织匀浆液(对于不超过60mg的组织样品,仅需加入100微升组织匀浆液),并在玻璃匀浆器内充分匀浆。
接下来请参照【细胞样品蛋白抽提】的步骤2)开始操作,即把沉淀当做已经离心收集好的细胞沉淀操作。(特别说明:对于起始量为60mg的组织样品,后续直接按照步骤2-7操作即可;对于起始量不超过60mg的组织样品,后续步骤中的溶液的用量须减半使用)。
对于组织样品,如果每个样品的重量不超过60mg,本试剂盒可以抽提50个样品,如果每个样品为60mg,本试剂盒可以抽提25个样品。
Q:该产品是否可以用于冻存组织?
A:对于组织样品,仅适合于新鲜组织,对冻存过的组织抽提效果很差。
Q:使用cocktail代替PMSF可以吗?
A:可以。
Q:这个试剂盒可以用来提取真菌或植物的细胞核膜蛋白吗?
A:本产品不适用于真菌及植物样品的。
Q:细胞沉淀收集后,如果不立马提蛋白,是需要放在-20还是-80保存吗?
A:建议提取蛋白后再保存。样本需要新鲜细胞的,不适合冻存的。
Q:该产品按说明书中所述,核在裂解之前,即使吸干净胞浆液体,会不会胞浆残留在核里面?
A:可以用预冷pbs洗一下,减少浆蛋白污染。
Published citations
[1] Li Z, Wu M, Liu S, et al. Apoptotic vesicles activate autophagy in recipient cells to induce angiogenesis and dental pulp regeneration [published online ahead of print, 2022 May 10]. Mol Ther. 2022;S1525-0016(22)00304-5. doi:10.1016/j.ymthe.2022.05.006(IF:11.454)
[2] Wei Y, Sun H, Zhang S, et al. Multi-walled carbon nanotubes inhibit potential detoxification of dioxin-mediated toxicity by blocking the nuclear translocation of aryl hydrocarbon receptor. J Hazard Mater. 2022;430:128458. doi:10.1016/j.jhazmat.2022.128458(IF:10.588)
[3] Zhao XB, Chen YP, Tan M, et al. Extracellular Matrix Stiffness Regulates DNA Methylation by PKCα-Dependent Nuclear Transport of DNMT3L. Adv Healthc Mater. 2021;10(16):e2100821. doi:10.1002/adhm.202100821(IF:9.933)
[4] Zhong W, Rao Z, Xu J, et al. Defective mitophagy in aged macrophages promotes mitochondrial DNA cytosolic leakage to activate STING signaling during liver sterile inflammation. Aging Cell. 2022;21(6):e13622. doi:10.1111/acel.13622(IF:9.304)
[5] Feng J, Dai W, Mao Y, et al. Simvastatin re-sensitizes hepatocellular carcinoma cells to sorafenib by inhibiting HIF-1α/PPAR-γ/PKM2-mediated glycolysis. J Exp Clin Cancer Res. 2020;39(1):24. Published 2020 Jan 30. doi:10.1186/s13046-020-1528-x(IF:7.068)
[6] Peng X, Guo H, Yuan J, et al. Extracellular vesicles released from hiPSC-derived MSCs attenuate chronic prostatitis/chronic pelvic pain syndrome in rats by immunoregulation. Stem Cell Res Ther. 2021;12(1):198. Published 2021 Mar 20. doi:10.1186/s13287-021-02269-x(IF:6.832)
[7] Weng J, Li S, Lin H, et al. PCDHGA9 represses epithelial-mesenchymal transition and metastatic potential in gastric cancer cells by reducing β-catenin transcriptional activity. Cell Death Dis. 2020;11(3):206. Published 2020 Mar 30. doi:10.1038/s41419-020-2398-z(IF:6.304)
[8] Sun Q, Yuan M, Wang H, et al. PKM2 Is the Target of a Multi-Herb-Combined Decoction During the Inhibition of Gastric Cancer Progression. Front Oncol. 2021;11:767116. Published 2021 Dec 2. doi:10.3389/fonc.2021.767116(IF:6.244)
[9] Sa R, Guo M, Liu D, Guan F. AhR Antagonist Promotes Differentiation of Papillary Thyroid Cancer via Regulating circSH2B3/miR-4640-5P/IGF2BP2 Axis. Front Pharmacol. 2021;12:795386. Published 2021 Dec 23. doi:10.3389/fphar.2021.795386(IF:5.811)
[10] Feng J, Wu L, Ji J, et al. PKM2 is the target of proanthocyanidin B2 during the inhibition of hepatocellular carcinoma. J Exp Clin Cancer Res. 2019;38(1):204. Published 2019 May 17. doi:10.1186/s13046-019-1194-z(IF:5.646)
[11] Weng J, Xiao J, Mi Y, et al. PCDHGA9 acts as a tumor suppressor to induce tumor cell apoptosis and autophagy and inhibit the EMT process in human gastric cancer. Cell Death Dis. 2018;9(2):27. Published 2018 Jan 18. doi:10.1038/s41419-017-0189-y(IF:5.638)
[12] Li Z, Jia Y, Han S, et al. Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6. Cell Physiol Biochem. 2018;47(6):2278-2290. doi:10.1159/000491538(IF:5.500)
[13] Yang N, Xiong Y, Wang Y, et al. ADAP Y571 Phosphorylation Is Required to Prime STAT3 for Activation in TLR4-Stimulated Macrophages. J Immunol. 2021;206(4):814-826. doi:10.4049/jimmunol.2000569(IF:5.422)
[14] Zhang X, Zhang B, Li L, Li X, Zhang J, Chen G. Fermented noni (Morinda citrifolia L.) fruit juice improved oxidative stress and insulin resistance under the synergistic effect of Nrf2/ARE pathway and gut flora in db/db mice and HepG2 cells [published online ahead of print, 2022 Jul 14]. Food Funct. 2022;10.1039/d2fo00595f. doi:10.1039/d2fo00595f(IF:5.396)
[15] Yu Q, Dai W, Ji J, et al. Sodium butyrate inhibits aerobic glycolysis of hepatocellular carcinoma cells via the c-myc/hexokinase 2 pathway. J Cell Mol Med. 2022;26(10):3031-3045. doi:10.1111/jcmm.17322(IF:5.310)
[16] Wang Y, Chen K, Cai Y, et al. Annexin A2 could enhance multidrug resistance by regulating NF-κB signaling pathway in pediatric neuroblastoma. J Exp Clin Cancer Res. 2017;36(1):111. Published 2017 Aug 16. doi:10.1186/s13046-017-0581-6(IF:5.189)
[17] Li T, Xiao G, Tan S, et al. HSF1 Attenuates LPS-Induced Acute Lung Injury in Mice by Suppressing Macrophage Infiltration. Oxid Med Cell Longev. 2020;2020:1936580. Published 2020 Dec 18. doi:10.1155/2020/1936580(IF:5.076)
[18] Yan YL, Huang ZN, Zhu Z, et al. Downregulation of TET1 Promotes Bladder Cancer Cell Proliferation and Invasion by Reducing DNA Hydroxymethylation of AJAP1. Front Oncol. 2020;10:667. Published 2020 May 21. doi:10.3389/fonc.2020.00667(IF:4.848)
[19] Li Z, Han S, Jia Y, et al. MCPIP1 regulates RORα expression to protect against liver injury induced by lipopolysaccharide via modulation of miR-155 [published online ahead of print, 2019 Feb 27]. J Cell Physiol. 2019;10.1002/jcp.28327. doi:10.1002/jcp.28327(IF:4.522)
[20] Zhang Y, Fang Z, Guo X, et al. lncRNA B4GALT1-AS1 promotes colon cancer cell stemness and migration by recruiting YAP to the nucleus and enhancing YAP transcriptional activity. J Cell Physiol. 2019;234(10):18524-18534. doi:10.1002/jcp.28489(IF:4.522)
[21] Han S, Li Z, Ji P, et al. MCPIP1 alleviated lipopolysaccharide-induced liver injury by regulating SIRT1 via modulation of microRNA-9. J Cell Physiol. 2019;234(12):22450-22462. doi:10.1002/jcp.28809(IF:4.522)
[22] Zhang XG, Liu AX, Zhang YX, et al. A diarylheptanoid compound from Alpinia officinarum Hance ameliorates high glucose-induced insulin resistance by regulating PI3K/AKT-Nrf2-GSK3β signaling pathways in HepG2 cells. J Ethnopharmacol. 2022;295:115397. doi:10.1016/j.jep.2022.115397(IF:4.360)
[23] Fan X, Shan X, Jiang S, et al. YAP promotes endothelial barrier repair by repressing STAT3/VEGF signaling. Life Sci. 2020;256:117884. doi:10.1016/j.lfs.2020.117884(IF:3.647)
[24] Han S, Li Z, Han F, et al. ROR alpha protects against LPS-induced inflammation by down-regulating SIRT1/NF-kappa B pathway. Arch Biochem Biophys. 2019;668:1-8. doi:10.1016/j.abb.2019.05.003(IF:3.559)
[25] Xi Z, Hu X, Chen X, et al. Protocatechuic acid exerts protective effects via suppression of the P38/JNK- NF-κB signalling pathway in an experimental mouse model of intracerebral haemorrhage. Eur J Pharmacol. 2019;854:128-138. doi:10.1016/j.ejphar.2019.03.008(IF:3.170)
[26] Zhang Y, Qiao B, Gao F, Wang H, Miao S, Zhao H. Melatonin protects H9c2 cells against ischemia/reperfusion‑induced apoptosis and oxidative stress via activation of the Nrf2 signaling pathway. Mol Med Rep. 2018;18(3):3497-3505. doi:10.3892/mmr.2018.9315(IF:2.952)
[27] Li Y, Qin G, Du J, Yue P, Zhang Y, Hou N. circRNA LDLRAD3 Enhances the Malignant Behaviors of NSCLC Cells via the miR-20a-5p-SLC7A5 Axis Activating the mTORC1 Signaling Pathway. J Healthc Eng. 2022;2022:2373580. Published 2022 Jan 6. doi:10.1155/2022/2373580(IF:2.682)
Human IgG 人免疫球蛋白G
36110ES
Bovine Serum Albumin(BSA), PCR Gra...
36100ES
SuperSignal MaxiSignal Maximum Se...
36224ES
Bovine Serum Albumin(BSA),Standard...
36101ES
Bovine Serum Albumin(BSA), IgG,Pro...
36103ES
Bovine Serum Albumin(BSA), Fatty A...
36104ES
CHAPS
20102ES
InStab™ Phosphatase Inhibitor Cock...
20109ES