BCA Protein Quantification Kit BCA蛋白浓度测定试剂盒（增强型）

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比色皿法微孔板法蛋白质研究

Product no.20201ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 20201ES76

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20201ES76 500 T（100 mL） ¥278.00促~~¥288.00~~ 现货 Enquire

20201ES86 2500 T（500 mL） ¥778.00促~~¥818.00~~ 现货 Enquire

20201ES90 5000 T（1000 mL） ¥1,518.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20201ES

Specification灵敏度高，最小检测蛋白量可达0.2 μg，检测浓度下限达到10 μg/mL。速度快，比一般的BCA蛋白浓度测定试剂盒显色所用时间短。比传统的Lowry法检测速度约快4倍。线性范围广，20-2000 μg/mL浓度范围内有较好的线性范围。不受

Origin翌圣生物科技(上海)股份有限公司

技术平台 比色皿法 微孔板法

应用方法 蛋白质研究用于BCA蛋白浓度测定

检测方法 BCA比色法

生物特性参数 蛋白类型牛血清白蛋白（BSA） 来源生物牛应用蛋白定量

技术参数规格比色皿法50次/250次/500次；酶标法500次/2500次/5000次 检测波长562 nm 灵敏度最小检测蛋白量0.2 μg，检测浓度下限10 μg/mL 线性范围20-2000 μg/mL 蛋白标准品浓度2 mg/mL 样品与工作液比例比色皿法1:20 (v/v)；微孔板法1:8 (v/v) 储存条件Part Ⅰ室温；Part Ⅱ -25~-15℃保存，有效期1年

实验类型 蛋白定量测定蛋白质浓度

FAQ

Q：产品有沉淀怎么办？

A：请 37ºC 温育并伴随搅拌促使其充分溶解。如发现细菌污染，则应丢弃，避免对实验结果造成影响。

Q：请问BCA 蛋白浓度测定时，待测样品是水溶液，可否用纯水直接溶解 BSA 蛋白标准品，而不用试剂盒里的 BSA 蛋白标准配制液？

A：BSA 溶解还是需要使用试剂盒自带的蛋白标准配制液，后续标准品的稀释可以使用水。

Q：该蛋白标准品 BSA 用什么稀释？

A：标准品稀释液为蛋白样品的溶解液，原则上，蛋白样品在什么溶液中，标准品也宜用什么溶液稀释。但也可用 0.9%的NaCl 或 1×PBS 进行稀释。

Q：试剂盒中的BSA 标准品可以另外购买吗？

A：可以的，我们公司提供有单独的 BSA 标准品（货号 20204）。

Q：每次测定时都需要做标准曲线吗？

A：是的，建议每次测定时都做标准曲线。因为BCA法测定时颜色会随着时间的延长不断加深，并且显色反应的速度和温度有关，所以除非精确控制显色反应的时间和温度，否则如需精确测定宜每次都做标准曲线。

Q：说明书中表2 BSA标准品体系配制为什么H的稀释总体积是50μL，其他都是30μL？

A：标准品浓度梯度的配置仅供参考，可以按照需要的浓度梯度进行配置。若按总体积30 μL稀释（1μL 2mg/mL的BSA原液，加29μL的稀释液）无法除尽，终浓度约为66.7 μg/mL 。

Documents & Citations

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Published citations

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