BCA Protein Quantification Kit BCA蛋白浓度测定试剂盒（增强型）

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比色皿法微孔板法蛋白质研究

Product no.20201ES Sizes3 options Documents1 files SourceYeasen · Official

Cat. No. 20201ES76

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20201ES76 500 T（100 mL） $278.00促~~$288.00~~ 现货 Enquire

20201ES86 2500 T（500 mL） $778.00促~~$818.00~~ 现货 Enquire

20201ES90 5000 T（1000 mL） $1,518.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No20201ES

Specification灵敏度高，最小检测蛋白量可达0.2 μg，检测浓度下限达到10 μg/mL。速度快，比一般的BCA蛋白浓度测定试剂盒显色所用时间短。比传统的Lowry法检测速度约快4倍。线性范围广，20-2000 μg/mL浓度范围内有较好的线性范围。不受

Origin翌圣生物科技(上海)股份有限公司

技术平台 比色皿法 微孔板法

应用方法 蛋白质研究用于BCA蛋白浓度测定

检测方法 BCA比色法

生物特性参数 蛋白类型牛血清白蛋白（BSA） 来源生物牛应用蛋白定量

技术参数规格比色皿法50次/250次/500次；酶标法500次/2500次/5000次 检测波长562 nm 灵敏度最小检测蛋白量0.2 μg，检测浓度下限10 μg/mL 线性范围20-2000 μg/mL 蛋白标准品浓度2 mg/mL 样品与工作液比例比色皿法1:20 (v/v)；微孔板法1:8 (v/v) 储存条件Part Ⅰ室温；Part Ⅱ -25~-15℃保存，有效期1年

实验类型 蛋白定量测定蛋白质浓度

FAQ

Q：产品有沉淀怎么办？

A：请 37ºC 温育并伴随搅拌促使其充分溶解。如发现细菌污染，则应丢弃，避免对实验结果造成影响。

Q：请问BCA 蛋白浓度测定时，待测样品是水溶液，可否用纯水直接溶解 BSA 蛋白标准品，而不用试剂盒里的 BSA 蛋白标准配制液？

A：BSA 溶解还是需要使用试剂盒自带的蛋白标准配制液，后续标准品的稀释可以使用水。

Q：该蛋白标准品 BSA 用什么稀释？

A：标准品稀释液为蛋白样品的溶解液，原则上，蛋白样品在什么溶液中，标准品也宜用什么溶液稀释。但也可用 0.9%的NaCl 或 1×PBS 进行稀释。

Q：试剂盒中的BSA 标准品可以另外购买吗？

A：可以的，我们公司提供有单独的 BSA 标准品（货号 20204）。

Q：每次测定时都需要做标准曲线吗？

A：是的，建议每次测定时都做标准曲线。因为BCA法测定时颜色会随着时间的延长不断加深，并且显色反应的速度和温度有关，所以除非精确控制显色反应的时间和温度，否则如需精确测定宜每次都做标准曲线。

Q：说明书中表2 BSA标准品体系配制为什么H的稀释总体积是50μL，其他都是30μL？

A：标准品浓度梯度的配置仅供参考，可以按照需要的浓度梯度进行配置。若按总体积30 μL稀释（1μL 2mg/mL的BSA原液，加29μL的稀释液）无法除尽，终浓度约为66.7 μg/mL 。

Documents & Citations

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Published citations

Chen P, Wang W, Liu R, et al. Olfactory sensory experience regulates gliomagenesis via neuronal IGF1. Nature. 2022;606(7914):550-556. doi:10.1038/s41586-022-04719-9(IF:49.962)
Miao Z, Li J, Wang Y, et al. Hsa_circ_0136666 stimulates gastric cancer progression and tumor immune escape by regulating the miR-375/PRKDC Axis and PD-L1 phosphorylation. Mol Cancer. 2023;22(1):205. Published 2023 Dec 13. doi:10.1186/s12943-023-01883-y (IF:37.3)
Liu Y, Liu Q, Zhao L, et al. Essential role of membrane vesicles for biological activity of the bacteriocin micrococcin P1. J Extracell Vesicles. 2022;11(4):e12212. doi:10.1002/jev2.12212(IF:25.841)
Liang J, Bi G, Huang Y, et al. MAFF confers vulnerability to cisplatin-based and ionizing radiation treatments by modulating ferroptosis and cell cycle progression in lung adenocarcinoma. Drug Resist Updat. 2024;73:101057. doi:10.1016/j.drup.2024.101057 (IF:24.3)
Xiao Y, Ma D, Yang YS, et al. Comprehensive metabolomics expands precision medicine for triple-negative breast cancer. Cell Res. 2022;32(5):477-490. doi:10.1038/s41422-022-00614-0(IF:25.617)
Li Y, Hu C, Zhai P, et al. Fibroblastic reticular cell-derived exosomes are a promising therapeutic approach for septic acute kidney injury. Kidney Int. 2024;105(3):508-523. doi:10.1016/j.kint.2023.12.007(IF:19.6)
Zhang Q, Shi D, Guo M, Zhao H, Zhao Y, Yang X. Radiofrequency-Activated Pyroptosis of Bi-Valent Gold Nanocluster for Cancer Immunotherapy. ACS Nano. 2023;17(1):515-529. doi:10.1021/acsnano.2c09242(IF:17.1)
Wang H, Shan X, Ren M, Shang M, Zhou C. Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis. Nucleic Acids Res. 2021;49(21):12306-12319. doi:10.1093/nar/gkab1121(IF:16.971)
Dai W, Tian R, Yu L, et al. Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma. Nat Commun. 2024;15(1):131. Published 2024 Jan 2 doi:10.1038/s41467-023-44576-2.(IF:16.6)
Hu X, Wang L, Wang Y, et al. RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins. Mol Cell. 2020;79(2):320-331.e9. doi:10.1016/j.molcel.2020.06.023(IF:15.584)
Yang M, Zheng X, Fan J, et al. Antibiotic-Induced Gut Microbiota Dysbiosis Modulates Host Transcriptome and m6A Epitranscriptome via Bile Acid Metabolism. Adv Sci (Weinh). Published online May 7, 2024. doi:10.1002/advs.202307981(IF:15.1)
Lu X, Chen X, Lin C, et al. Elesclomol Loaded Copper Oxide Nanoplatform Triggers Cuproptosis to Enhance Antitumor Immunotherapy. Adv Sci (Weinh). 2024;11(18):e2309984. doi:10.1002/advs.202309984(IF:15.1)
Li L, Zeng X, Chao Z, et al. Targeting Alpha-Ketoglutarate Disruption Overcomes Immunoevasion and Improves PD-1 Blockade Immunotherapy in Renal Cell Carcinoma. Adv Sci (Weinh). 2023;10(27):e2301975. doi:10.1002/advs.202301975(IF:15.1)
Weng Q, Sun H, Fang C, et al. Catalytic activity tunable ceria nanoparticles prevent chemotherapy-induced acute kidney injury without interference with chemotherapeutics. Nat Commun. 2021;12(1):1436. Published 2021 Mar 4. doi:10.1038/s41467-021-21714-2(IF:14.919)
Zhao H, Xu J, Huang W, et al. Spatiotemporally Light-Activatable Platinum Nanocomplexes for Selective and Cooperative Cancer Therapy. ACS Nano. 2019;13(6):6647-6661. doi:10.1021/acsnano.9b00972(IF:13.903)
Weng Q, Hu X, Zheng J, et al. Toxicological Risk Assessments of Iron Oxide Nanocluster- and Gadolinium-Based T1MRI Contrast Agents in Renal Failure Rats. ACS Nano. 2019;13(6):6801-6812. doi:10.1021/acsnano.9b01511(IF:13.903)
Liu C, Sun W, Zhu T, et al. Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy. Redox Biol. 2022;52:102292. doi:10.1016/j.redox.2022.102292(IF:11.799)
Gao X, You J, Gong Y, et al. WSB1 regulates c-Myc expression through β-catenin signaling and forms a feedforward circuit. Acta Pharm Sin B. 2022;12(3):1225-1239. doi:10.1016/j.apsb.2021.10.021(IF:11.614)
Hu Q, Jia L, Zhang X, Zhu A, Wang S, Xie X. Accurate construction of cell membrane biomimetic graphene nanodecoys via purposeful surface engineering to improve screening efficiency of active components of traditional Chinese medicine. Acta Pharm Sin B. 2022;12(1):394-405. doi:10.1016/j.apsb.2021.05.021(IF:11.614)
Xu X, Shi Y, Luan P, et al. The subcellular redistribution of NLRC5 promotes angiogenesis via interacting with STAT3 in endothelial cells. Theranostics. 2021;11(9):4483-4501. Published 2021 Mar 4. doi:10.7150/thno.54473(IF:11.556)
Yao C, Ni Z, Gong C, et al. Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy. Autophagy. 2018;14(10):1831-1844. doi:10.1080/15548627.2018.1489946(IF:11.100)
Dou Y, Xie J, Tan Y, Zhang M, Zhao Y, Liu X. Neurotransmitter-stimulated neuron-derived sEVs have opposite effects on amyloid β-induced neuronal damage. J Nanobiotechnology. 2021;19(1):324. Published 2021 Oct 15. doi:10.1186/s12951-021-01070-5(IF:10.435)