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Product data sheet PD-21101ES

Alkaline Phosphatase Activity Detection Kit 碱性磷酸酶活性检测试剂盒

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Alkaline Phosphatase Activity Detection Kit 碱性磷酸酶活性检测试剂盒

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比色分析法干细胞研究肿瘤研究肝胆疾病研究骨骼疾病研究蛋白质分析

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Cat. No. 21101ES60 现货 ¥856.00

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No21101ES

Specification100 T

Origin翌圣生物科技(上海)股份有限公司

技术平台 比色分析法

应用方法 干细胞研究ALP作为iPS细胞成功诱导的标志 肿瘤研究用于结肠癌细胞分化程度的定性和定量指标 肝胆疾病研究血清ALP活性升高与胆管阻塞、肝硬化、肝癌等肝胆疾病相关 骨骼疾病研究ALP活性与骨骼损伤和骨骼疾病相关 蛋白质分析蛋白质分析相关试剂

适用物种种属通用

检测方法 吸光度检测（405 nm）

生物特性参数 蛋白类型碱性磷酸酶（ALP/AKP） 表达/来源系统通用（无特定种属限制） 应用检测细胞或组织裂解液/匀浆液、血清、血浆、尿液、纯化酶等样品中的ALP活性

样本类型 细胞裂解液 组织匀浆液 血清血浆尿液 纯化酶

技术参数 储存条件-20℃保存，一年有效；显色底物、pNP标准品需避光保存 运输条件冰袋运输 规格100T 检测波长405 nm 检测原理pNPP在碱性条件下被ALP水解生成黄色pNP，405 nm处有最大吸收峰 显色底物对硝基苯磷酸（pNPP） 标准品pNP标准品溶液（10 mM） 制剂配方ALP反应缓冲液、pNPP底物、pNP标准品溶液、反应终止液

实验类型 碱性磷酸酶活性检测利用pNPP显色底物检测样本中ALP活性 比色定量分析通过OD405吸光值结合pNP标准品定量计算ALP活性

FAQ

Q：为什么该试剂盒检测不准？

A：可能是试剂盒各个成分没有恢复到室温即使用。

Q：在结果检测时 405nm 处测不到吸光值，怎么办？

A：如果不能测定 405nm，也可以在 400-415nm 范围内检测吸光度。如果不能立即测定，可以在数小时内完成测定，所显现的黄色在数小时（≤ 6h）内稳定。

Q：37℃孵育 5min，为什么检测不出来酶活？

A：建议延长孵育时间至 30min。

Q：该试剂盒中的产品可以直接在室温下操作的吗？

A：ALP 反应缓冲液和 p-nitrophenol 标准品溶液对人体有害，请注意适当防护。反应终止液有腐蚀性，请小心操作。最好在通风橱里操作。

Q：该试剂盒按说明书检测完后如何计算酶活？

A：首先，绘制标曲：0.5mM*标准品的用量分别为4、8、16、24、32和40微升，用微摩尔数做横坐标，标曲方程可通过Excel做；然后，根据标准曲线及待检测样品的吸光度值，可以计算得到样品中p-nitrophenol生成量。再根据酶活性定义进行计算。即p-nitrophenol的微摩尔数除以孵育时间（min）。计算出的酶活单位用U表示。最后，样品的酶活浓度可以用酶活/（上样体积*蛋白浓度），最后可以表示成U/mg。

Documents & Citations

Published citations

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