HiActi® Recombinant Non-biotinylated Human IL-4 Protein,His-Avi Tag重组人白介素-4

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HEK293细胞表达系统通用

Product no.90326ES Sizes3 options SourceYeasen · Official

Cat. No. 90326ES25

现货 $1,185.00

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Cat.No Spec Price Status

90326ES25 25 μg $1,185.00 现货 Enquire

90326ES60 100 μg $4,735.00 现货 Enquire

90326ES76 500 μg $15,755.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90326ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法通用作为细胞因子用于免疫学相关研究

适用物种种属人

检测方法 SDS-PAGE LAL ELISA 细胞增殖实验

生物特性参数 蛋白类型重组蛋白 种属来源人 (Human) 生物活性描述IL-4能刺激活化B细胞和T细胞增殖；基于TF-1细胞增殖实验的ED50<0.2 ng/mL 应用可用于ELISA、细胞增殖实验等免疫学研究

技术参数纯度>95% as determined by SDS-PAGE 内毒素水平Less than 1 EU per μg by the LAL method 分子量预测17.8 kDa；糖基化后SDS-PAGE迁移至23-25 kDa 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ED50 <0.2 ng/mL (cell proliferation assay using TF-1 cells) 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月；避免反复冻融 表达系统HEK293 标签C-His-Avi 表达序列His25-Ser153 Accession号P05112 复溶方法用蒸馏水溶解冻干蛋白；建议复溶浓度0.5 mg/mL(25-100μg)或1 mg/mL(500μg-1mg)；避免剧烈涡旋