HiActi® Recombinant Sus scrofa IL-22 Protein,His Tag

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HEK293细胞表达系统上皮稳态与屏障功能研究

Product no.90351ES Sizes3 options SourceYeasen · Official

Cat. No. 90351ES25

现货 $1,295.00

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90351ES25 25 μg $1,295.00 现货 Enquire

90351ES60 100 μg $5,185.00 现货 Enquire

90351ES76 500 μg $17,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90351ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 上皮稳态与屏障功能研究研究IL-22在上皮细胞生长、通透性、黏液及抗菌蛋白产生等方面的功能

适用物种种属猪

检测方法 SDS-PAGE SEC-HPLC ELISA 鲎试剂法（LAL）

生物特性参数 蛋白类型重组猪IL-22（Interleukin-22） 种属来源猪（Porcine） 生物活性描述ELISA Data 1:Immobilized Sus scrofa IL-22 His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-22R alpha 1 hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. 应用上皮稳态与屏障功能研究

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量The protein has a predicted MW of 19.77 kDa. Due to glycosylation the protein migrates to 35-45 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ELISA Data 1:Immobilized Sus scrofa IL-22 His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-22R alpha 1 hFc Tag with the EC50 of 0.12μg/ml determined by ELISA . 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后， -85~-65℃保存，3个月有效期。避免反复冻融。 表达序列Val34-Asn190 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。 AccessionF1SH68 表达系统HEK293 标签C-His 外观Lyophilized Powder