HiActi® Recombinant Human IL-37 Protein 重组人白介素-37

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大肠杆菌表达系统炎症研究

Product no.90373ES Sizes4 options SourceYeasen · Official

Cat. No. 90373ES08

现货 ¥535.00

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90373ES08 5μg ¥535.00 现货 Enquire

90373ES25 25 μg ¥1,335.00 现货 Enquire

90373ES50 50μg ¥2,135.00 现货 Enquire

90373ES60 100μg ¥3,845.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No90373ES

Specification高活性、高纯度、低内毒素、高稳定性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

应用方法 炎症研究IL-37作为抗炎细胞因子，用于研究炎症反应机制及相关疾病模型

适用物种种属人

检测方法 SDS-PAGE LAL法

生物特性参数 蛋白类型重组人白细胞介素-37 (IL-37) 种属来源人 生物活性描述抗炎性细胞因子，能够限制炎症反应，保护小鼠免于结肠炎和LPS诱导的休克；与IL18BP结合抑制IL18活性；细胞内与Smad3结合转位至细胞核减少促炎细胞因子分泌。应用炎症相关疾病模型研究

技术参数纯度> 98% as determined by SDS-PAGE. 内毒素水平< 0.01 EU/μg of rHuIL-37 protein as determined by LAL method. 分子量Approximately 18.6 kDa a single non-glycosylated polypeptide chain containing 166 amino acids. 制剂形式Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB with 150 mM NaCl pH 7.4. 活性Fully biologically active when compared to standard. When recombinant human (rh) IL18 R alpha/IL1 R5 Fc Chimera is immobilized at 2 μg/mL (100 μL/well) the concentration of rhIL-37 that produces 50% optimal binding response is 20-120 ng/ml. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，2~8℃保存，7天有效期。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统E.coli 标签No Tag 复溶方法开盖之前，先离心，以将内容物带到底部。使用无菌蒸馏水复溶，重新配制至0.1-1.0 mg/mL的浓度，可根据后续实验更进一步稀释；长期储存，建议稀释缓冲液中加入载体蛋白(0.1% BSA)；分装为一次实验用量，-80℃冻存，避免反复冻融。外观Lyophilized Powder 表达序列Lys27-Asp192 Accession号NP775297