HiActi® Recombinant Rat PDGF-AA Protein,His Tag 重组大鼠血小板衍生生长因子-AA

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酵母表达系统

Product no.91608ES Sizes3 options SourceYeasen · Official

Cat. No. 91608ES25

现货 $1,885.00

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Cat.No Spec Price Status

91608ES25 25 μg $1,885.00 现货 Enquire

91608ES60 100 μg $5,655.00 现货 Enquire

91608ES76 500 μg $16,965.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No91608ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 酵母表达系统

适用物种种属大鼠

检测方法 SDS-PAGE Bis-Tris PAGE LAL法 细胞增殖实验

生物特性参数 蛋白类型重组细胞因子/血小板衍生生长因子 种属来源大鼠 生物活性描述Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 5 - 15 ng/mL. 应用调节细胞生长和分裂；与PDGFR-αα结合后刺激成骨细胞增殖，可能在调节骨形成过程中发挥自分泌和旁分泌作用

技术参数纯度>90% as determined by Bis-Tris PAGE 内毒素水平<1.0 EU per μg by the LAL method 分子量Predicted MW 14.75 kDa; migrates to 17-22 kDa and 25-45 kDa due to glycosylation on Bis-Tris PAGE 制剂形式Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ED50 for cell proliferation is 5-15 ng/mL 储存条件-25~-15℃保存，有效期1年；复溶后-85~-65℃保存，3个月有效期 表达序列Arg86-Lys204 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 表达系统Yeast 标签N-His 外观Lyophilized Powder AccessionP28576-1