HiActi® Recombinant Human HGF Protein 重组人肝细胞生长因子

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CHO细胞表达系统类器官培养原代细胞培养干细胞定向分化信号通路研究疾病模型构建

Product no.92055ES Sizes5 options SourceYeasen · Official

Cat. No. 92055ES80

现货 ¥11,195.00

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Cat.No Spec Price Status

92055ES80 1 mg ¥11,195.00 现货 Enquire

92055ES10 10 μg ¥388.00促~~¥485.00~~ 现货 Enquire

92055ES50 50 μg ¥1,348.00促~~¥1,685.00~~ 现货 Enquire

92055ES60 100 μg ¥2,148.00促~~¥2,685.00~~ 现货 Enquire

92055ES76 500 μg ¥6,715.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No92055ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 CHO细胞表达系统

应用方法 类器官培养构建与维持肝/肾类器官 原代细胞培养支持原代肝细胞/胆管细胞/肾小管上皮细胞培养与扩增 干细胞定向分化诱导胚胎干细胞、iPSC、间充质干细胞肝向/肾向分化 信号通路研究激活HGF/c-Met、PI3K/Akt、MAPK等通路 疾病模型构建构建肝/肾损伤、纤维化、肿瘤转移模型

适用物种种属人

检测方法 SDS-PAGE LAL法

生物特性参数 蛋白类型生长因子 种属来源人 生物活性描述ED50 < 10.0 ng/ml measured in a cell proliferation assay using 4MBr5 cells corresponding to a specific activity of > 1.0 × 10^5 units/mg. 应用类器官培养、原代细胞培养、干细胞定向分化、信号通路研究、疾病模型构建 产品别称Scatter Factor (SF) Hepatopoietin (HPTA)

技术参数纯度> 95% as determined by SDS-PAGE. 内毒素水平< 0.2 EU per μg by the LAL method. 分子量88~90 kDa (single chain) 59~61 kDa (alpha chain) 30~34 kDa (beta chain) on SDS-PAGE under reducing conditions. 制剂形式Lyophilized after extensive dialysis against PBS. 活性ED50 < 10.0 ng/ml specific activity > 1.0 × 10^5 units/mg. 储存条件-85~-65℃保存，有效期1年；复溶后2~8℃保存7天，-25~-15℃保存3个月；建议分装避免反复冻融。 表达系统CHO 标签No Tag 表达序列Gln32-Ser728 登录号P14210 外观Lyophilized Powder 复溶方法短暂离心后，用ddH2O或PBS复溶至100 μg/ml；长期储存建议添加0.1% BSA。

实验类型 SDS-PAGE纯度检测 细胞增殖实验生物活性测定

Documents & Citations

Published citations

[1]. Shenghong W, Peilan G, Wenqiang M, et al. Liquid-liquid phase separation of ATXN2L enhances mRNA translation in hepatocellular carcinoma. Cell Reports,2025. https://doi.org/10.1016/j.celrep. (IF=9.9)