Recombinant Human MSLN/Mesothelin (hFc Tag)

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HEK293表达系统CAR-T肿瘤研究

Product no.93101ES Sizes4 options SourceYeasen · Official

Cat. No. 93101ES20

现货 ¥985.00

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Cat.No Spec Price Status

93101ES20 20 μg ¥985.00 现货 Enquire

93101ES60 100 μg ¥4,445.00 现货 Enquire

93101ES76 500 μg ¥14,855.00 现货 Enquire

93101ES80 1 mg ¥28,225.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93101ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 CAR-T靶点蛋白 肿瘤研究研究肿瘤细胞增殖、迁移及肿瘤进展

适用物种种属人

检测方法 LAL法 SDS-PAGE HPLC ELISA

生物特性参数 蛋白类型重组蛋白/靶点蛋白 种属来源人 生物活性描述1. Immobilized Human CA125 His Tag at 2μg/mL(100μL/Well) on the plate. Dose response curve for Human MSLN hFc Tag with the EC50 of 2.8μg/mL determined by ELISA. 2. Immobilized Human MSLN at 0.5μg/mL(100μL/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody hFc Tag with the EC50 of 29.2ng/mL determined by ELISA. 应用CAR-T靶点蛋白；肿瘤研究

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Approximately 59.0 kDa. Due to glycosylation the protein migrates to 68-78 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性1. ELISA: Immobilized Human CA125 His Tag at 2μg/mL (100μL/Well) EC502.8 μg/mL for Human MSLN hFc Tag. 2. ELISAImmobilized Human MSLN at 0.5μg/mL (100μL/well) EC5029.2 ng/mL for Biotinylated Anti-MSLN Antibody hFc Tag. 储存条件-25 ~ -15℃保存，收到货后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签C-hFc 外观Lyophilized Powder AccessionQ13421-2 表达序列Glu296-Asp580 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。