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Product data sheet PD-93545ES
Recombinant Human B7-1/CD80 (hFc Tag) Recombinant Human B7-1/CD80 (hFc Tag)
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Recombinant Human B7-1/CD80 (hFc Tag)

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HEK293细胞表达系统免疫检查点研究共刺激通路研究
Product no.93545ES Sizes4 options SourceYeasen · Official
Cat. No. 93545ES20
现货 ¥985.00
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Cat.No Spec Price Status
93545ES20 20 μg ¥985.00 现货 Enquire
93545ES60 100 μg ¥3,385.00 现货 Enquire
93545ES76 500 μg ¥11,305.00 现货 Enquire
93545ES80 1 mg ¥21,475.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No93545ES
Specification高纯度、高活性、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
技术平台 HEK293细胞表达系统
应用方法 免疫检查点研究B7-1/CD80是共刺激通路关键分子,用于免疫调节研究 共刺激通路研究调控T/B细胞反应
适用物种种属 人
检测方法 ELISA SDS-PAGE HPLC LAL法
生物特性参数 蛋白类型重组蛋白/靶点蛋白/免疫检查点 种属来源Human 生物活性描述Immobilized Human B7-1 hFc Tag at 0.5μg/ml (100μL/Well) on the plate. Dose response curve for Human CTLA-4 His Tag with the EC50 of 66ng/ml determined by ELISA 应用B7-1/CD80与B7-2/CD86及其受体CD28和CTLA-4构成共刺激通路,调节T/B细胞反应;CTLA-4与B7-1结合亲和力高于CD28,参与免疫反应下调
技术参数 纯度>95% as determined by SDS-PAGE and HPLC 内毒素水平<1.0 EU per μg by the LAL method 分子量Predicted MW 50.5 kDa migrates to 70-80 kDa due to glycosylation (Tris-Bis PAGE) 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization 活性ELISA Data: Immobilized Human B7-1 hFc Tag at 0.5μg/ml (100μL/Well) on the plate. Dose response curve for Human CTLA-4 His Tag with the EC50 of 66ng/ml determined by ELISA 储存条件-25 ~ -15℃保存,收到货后有效期1年;复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期 表达序列Val35-Asn242 表达系统HEK293 标签C-hFc 复溶方法开盖前先离心;使用蒸馏水复溶冻干蛋白,复溶浓度超过100 μg/mL;建议首次使用时分装,避免反复冻融 外观Lyophilized Powder AccessionP33681-1 产品别称CD80 molecule B7 B71 B7-1 BB1 CD28LG1 CD80 B7.1 CD28LG1 LAB7
实验类型 ELISA用于检测生物活性 SDS-PAGE用于检测纯度 HPLC用于检测纯度 Tris-Bis PAGE用于检测分子量 LAL法用于检测内毒素水平

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