Recombinant Cynomolgus PD-L1/B7-H1 Protein,His Tag

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HEK293表达系统免疫检查点研究抗体筛选

Product no.93649ES Sizes3 options SourceYeasen · Official

Cat. No. 93649ES60

现货 ¥3,585.00

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93649ES60 100 μg ¥3,585.00 现货 Enquire

93649ES76 500 μg ¥12,355.00 现货 Enquire

93649ES25 25 μg ¥1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93649ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 免疫检查点研究PD-L1/B7-H1靶点研究 抗体筛选抗PD-L1抗体的ELISA结合活性筛选

适用物种种属 食蟹猴

检测方法 SDS-PAGE HPLC ELISA LAL法

生物特性参数 蛋白类型重组蛋白/靶点蛋白/免疫检查点蛋白 种属来源食蟹猴(Cynomolgus) 生物活性描述ELISA验证：Cynomolgus/Rhesus macaque PD-L1 His Tag包被于板(1μg/ml 100μl/Well)，抗PD-L1抗体(hFc Tag)剂量反应曲线EC506.2 ng/ml。 应用免疫检查点研究、抗PD-L1抗体筛选

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 26 kDa; due to glycosylation migrates to 35-45 kDa by SDS-PAGE. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ELISA: EC50 of 6.2 ng/ml for Anti-PD-L1 Antibody (hFc Tag) binding to immobilized Cynomolgus/Rhesus macaque PD-L1 His Tag. 储存条件-25 ~ -15℃保存，收到货后有效期1年；复溶后，无菌条件下-85 ~ -65℃保存，3个月有效期。 表达序列Phe19-Arg238 表达系统HEK293 标签C-His AccessionG7PSE7 外观冻干粉末 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 产品别称PD-L1 PDL1 CD274 B7-H1