Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein,His-Avi Tag

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大肠杆菌表达系统抗体药物靶点研究配体-受体结合分析

Product no.95987ES Sizes3 options SourceYeasen · Official

Cat. No. 95987ES25

现货 ¥2,155.00

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95987ES25 25 μg ¥2,155.00 现货 Enquire

95987ES60 100 μg ¥7,185.00 现货 Enquire

95987ES76 500 μg ¥21,695.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No95987ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 大肠杆菌表达系统

应用方法 抗体药物靶点研究靶点蛋白 配体-受体结合分析评估IL-1β与IL-1R2的结合活性

适用物种种属小鼠

检测方法 SDS-PAGE HPLC ELISA LAL法

生物特性参数 蛋白类型重组生物素化小鼠IL-1 Beta/IL-1F2蛋白，His-Avi标签 种属来源小鼠 生物活性描述ELISA数据显示：包被Biotinylated Mouse IL-1 Beta His Tag后，Human IL-1 R2 hFc Tag的EC50为25.4 ng/mL；包被Mouse IL-1R2 His Tag后，Biotinylated Mouse IL-1 Beta His Tag的EC50为1.2 μg/mL。 应用抗体药物靶点研究；配体-受体结合分析

技术参数纯度>95% by SDS-PAGE and HPLC 内毒素水平<1.0 EU/μg by LAL method 分子量预测分子量20.30 kDa；Bis-Tris PAGE迁移至20-25 kDa 表达序列Val118-Ser269 制剂形式0.22 μm过滤PBS (pH 7.4)溶液冻干，冻干前加入8%海藻糖保护剂 活性ELISA EC50: 25.4 ng/mL (Human IL-1 R2 hFc Tag)；1.2 μg/mL (Mouse IL-1R2 His Tag) 储存条件-25~-15℃保存，有效期1年；复溶后在无菌条件下-85~-65℃保存3个月 外观冻干粉 (Lyophilized Powder) 表达系统E.coli 标签N-His-Avi 登录号P10749 复溶方法开盖前先离心；用蒸馏水复溶冻干蛋白，复溶浓度超过100 μg/mL；建议分装使用，避免反复冻融