Recombinant Biotinylated SARS-COV-2 Spike S1 Protein,His-Avi Tag

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HEK293细胞表达系统新冠相关研究受体结合研究

Product no.96478ES Sizes3 options SourceYeasen · Official

Cat. No. 96478ES25

现货 ¥1,975.00

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96478ES25 25 μg ¥1,975.00 现货 Enquire

96478ES60 100 μg ¥7,885.00 现货 Enquire

96478ES76 500 μg ¥26,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No96478ES

Specification高活性、高纯度、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 新冠相关研究研究SARS-CoV-2 Spike蛋白与ACE2受体的结合机制 受体结合研究评估S1蛋白与ACE2受体的结合活性

适用物种种属 SARS-CoV-2

检测方法 SDS-PAGE SEC-HPLC 鲎试剂法（LAL） 酶联免疫吸附试验（ELISA）

生物特性参数 蛋白类型重组蛋白 种属来源SARS-CoV-2 生物活性ELISA Data 1: Immobilized Biotinylated SARS-COV-2 Spike S1 His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2 hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and SEC-HPLC 内毒素水平Less than 1 EU per μg by the LAL method. 分子量The protein has a predicted MW of 77.9 kDa. Due to glycosylation the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25~-15℃保存，收到货之日起，有效期1年。复溶后，-85~-65℃保存，3个月有效期。避免反复冻融。 复溶方法用蒸馏水溶解冻干的蛋白。如果规格为25μg或者100μg，建议复溶到0.5mg/mL的浓度。对于500μg或1mg的冻干粉，建议以1mg/mL的浓度复溶。复溶过程中请不要剧烈涡旋或者剧烈摇晃。将复溶后的蛋白溶液按照需求进行分装，储存于至-80℃，避免反复冻融。 表达序列Gln14-Arg683 表达系统HEK293 标签C-His-Avi 外观Lyophilized Powder AccessionQHD43416.1 产品别称S1 protein;Spike protein S1;Spike S1 protein;S glycoprotein Subunit1