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Product data sheet PD-15070ES
FuniCut® BbsI FuniCut® BbsI
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FuniCut® BbsI

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质粒线性化载体构建快速DNA切割
Product no.15070ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15070ES25 现货 $158.00 促$165.00
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15070ES25 25 T $158.00促$165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15070ES
Specification识别位置5'-GAAGAC(N)₂↓-3'3'-CTTCTG(N)₆↑-5'推荐反应条件1×FuniCut® 缓冲液;37°C孵育酶活20 U/μL失活条件80℃温育20 min同裂酶BstV2I, BpiI甲基化修饰影响Dam、Dcm、C
Origin翌圣生物科技(上海)股份有限公司
应用方法 质粒线性化用于获得线性化质粒DNA 载体构建用于DNA片段克隆和载体构建 快速DNA切割用于快速切割质粒DNA、PCR产物、基因组DNA等
生物特性参数 试剂类型限制性内切酶 应用质粒线性化、载体构建、快速切割质粒DNA、PCR产物、基因组DNA等
样本类型 质粒DNA PCR产物 基因组DNA
技术参数 浓度20 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 识别位点5'-GAAGAC(N)₂↓-3' / 3'-CTTCTG(N)₆↑-5' 推荐反应条件1×FuniCut® 缓冲液;37°C孵育 失活条件80℃温育20 min 同裂酶BstV2I BpiI 甲基化修饰影响Dam、Dcm、CpG、EcoKI、EcoBI:无影响
实验类型 限制性内切酶酶切特异性识别DNA序列并切割双链DNA

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