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3ans

314views

Which brands of PBS, trypsin, and dual antibiotics are commonly used in cell culture rooms?

Supplier & Sourcing LLisaAssay 2026-03-30 #Cell culture #Common reagents #Pancreatin

4ans

192views

For animal cell mycoplasma detection PCR, should prefabricated agarose gels or self-prepared ones be used?

Reagent Selection 孙孙博杰721 2026-07-10 #Agarose #Precast gel #Mycoplasma testing

4ans

239views

For cell culture, if you want to check for mycoplasma, how much does Giemsa staining differ in effectiveness from fluorescence methods?

Lab Techniques 肖肖阿宝6830 2026-04-09 #Cell culture #Mycoplasma testing #Giemsa staining

4ans

304views

When purchasing large quantities of dual antibiotics for animal facilities, how should one choose between domestic and imported options considering lead time and price?

Compliance 冯冯研发 2026-03-03 #Penicillin-Streptomycin #Procurement price comparison #Lead time

4ans

195views

Will adding penicillin-streptomycin to primary rat fibroblasts affect their growth?

Storage & Shipping RReagenting8982 2026-09-07 #Penicillin-Streptomycin #Primary cell culture #Cytotoxicity

4ans

260views

What is the appropriate safe concentration of DMSO as a solvent for intraperitoneal injection in animal experiments using mice?

Lab Techniques 郑郑杰7662 2026-07-31 #Dimethyl sulfoxide #Animal experiments #Intraperitoneal injection

4ans

231views

Can the same batch of PBS used for culturing cells and raising animals in the laboratory be shared?

Compliance RResearchHub8536 2026-09-15 #Phosphate buffer solution #Cell culture #Animal experiments

4ans

234views

What should be added to DMEM when isolating lymphocytes from rat spleen for culture? What is the lead time?

Medical Devices LLily 2026-06-27 #DMEM medium #Primary cell culture #Rat spleen

4ans

234views

Can fetal bovine serum still be used for animal cell culture after repeated freeze-thaw cycles?

Supplier & Sourcing GGenePro 2026-09-15 #Fetal Bovine Serum #Repeated freeze-thaw #Cell culture

4ans

207views

Question: Can domestic fetal bovine serum be used as a substitute for imported fetal bovine serum in veterinary vaccine production?

Compliance SSam8092 2026-04-05 #Fetal Bovine Serum #Veterinary vaccine #Domestic substitution

4ans

200views

For culturing HepG2 and RAW264.7, should DMEM high glucose or 1640 be used? Is glutamine supplementation needed?

Lab Techniques AAndyProtein 2026-04-25 #Cell culture medium #DMEM #RPMI1640

Recombinant SARS-COV...

Recombinant SARS-Cov...

4ans

226views

Which brand of serum-free medium for mesenchymal stem cells is everyone using? Please compare prices and lead times.

Reagent Selection 王王倩文 2026-07-14 #Cell culture medium #Mesenchymal stem cells #Serum-free

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