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5ans

218views

Seeking advice on choosing AAV packaging transfection reagents: can they be used for both adherent 293 and suspension 293 cells?

Lab Techniques 邓邓燕杰1399 2026-08-28 #AAV packaging #Transfection reagent #293T

5ans

161views

Which transfection reagent offers high efficiency and low cytotoxicity for transfecting plasmids into HEK293T cells?

Reagent Selection 黄黄宇569 2026-09-11 #Transfection reagent #HEK293T #Lentiviral packaging

4ans

150views

CRISPR library screening for tumor drug resistance genes: How to solve the issue of low lentiviral packaging titer?

Storage & Shipping 刘刘涛阳2427 2026-09-23 #CRISPR library #Lentiviral packaging #293T

4ans

194views

For monoclonal screening after CRISPR editing, is limiting dilution or flow cytometry sorting more reliable? How many cells are needed for T7E1?

Reagent Selection 梁梁伟 2026-06-20 #CRISPR #Monoclonal screening #T7E1

5ans

183views

Transfection reagent for AAV packaging: How can the transfection efficiency of the three-plasmid system in HEK293T be improved?

Medical Devices KKevinBuffer4446 2026-09-16 #AAV packaging #HEK293T #Three-plasmid

5ans

212views

For lentiviral packaging in 293T cells, which transfection reagent offers high cost-effectiveness and stable lead times?

Lab Techniques 曹曹晨8654 2026-09-19 #Lentiviral packaging #293T #Transfection reagent

4ans

234views

Protein extraction biological reagent AB always forms precipitate, is it because the concentration is incorrect?

Compliance 蔡蔡采购 2026-08-14 #Protein purification #Biological reagent AB #Precipitation

3ans

209views

What should I do if the 293T cell status deteriorates after changing to a new batch of fetal bovine serum

Reagent Selection LLisa5508 2026-07-07 #Fetal Bovine Serum #Batch-to-batch variation #293T

3ans

270views

Is it normal for there to be significant differences in COA data among different batches of fetal bovine serum from the same brand?

Supplier & Sourcing 罗罗抗体 2026-07-12 #Fetal Bovine Serum #COA interpretation #Inter-batch variation

3ans

252views

Is the significant difference in flocculent precipitation of fetal bovine serum from different batches a quality issue

Compliance 细细胞同学3992 2026-07-01 #Fetal Bovine Serum #Inter-batch variation #Flocculent precipitate

3ans

290views

Will the fluctuation of endotoxin levels in fetal bovine serum from different batches of the same brand affect 293T transfection?

Compliance 韩韩敏敏 2026-08-05 #Fetal Bovine Serum #Batch-to-batch variation #Endotoxin testing

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