TechniquesCCK-8，科研实验中最常见的细胞增殖检测方法

实验原理

实验流程和操作步骤

01

实验分组（每组建议3-6个复孔）

02

实验方案

结果分析

文献示例一——计算细胞存活率

文献示例二——直接展示细胞OD值

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引用YEASEN产品部分发表文献参考

[1] Sun L, Li P, Ju X, et al. In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Cell. 2021;184(7):1865-1883.e20. doi:10.1016/j.cell.2021.02.008(IF:41.584)

[2] Wei S, Zhao Q, Zheng K, et al. GFAT1-linked TAB1 glutamylation sustains p38 MAPK activation and promotes lung cancer cell survival under glucose starvation. Cell Discov. 2022;8(1):77. Published 2022 Aug 9. doi:10.1038/s41421-022-00423-0(IF:38.079)

[3] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293. doi:10.1038/s41587-019-0249-1(IF:31.864)

[4] Yang F, Xiao Y, Ding JH, et al. Ferroptosis heterogeneity in triple-negative breast cancer reveals an innovative immunotherapy combination strategy [published online ahead of print, 2022 Oct 11]. Cell Metab. 2022;S1550-4131(22)00411-9. doi:10.1016/j.cmet.2022.09.021(IF:31.373)

[5] Rong QX, Wang F, Guo ZX, et al. GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma. Mol Cancer. 2021;20(1):80. Published 2021 May 29. doi:10.1186/s12943-021-01374-y(IF:27.401)

[6] Xia B, Shen X, He Y, et al. SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4(IF:25.617)

[7] Yang X, Zhao X, Zhu Y, et al. FBXO34 promotes latent HIV-1 activation by post-transcriptional modulation. Emerg Microbes Infect. 2022;11(1):2785-2799. doi:10.1080/22221751.2022.2140605(IF:19.568)

[8] Zhou Z, Zhang X, Lei X, et al. Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Signal Transduct Target Ther. 2021;6(1):382. Published 2021 Nov 3. doi:10.1038/s41392-021-00800-3(IF:18.187)

[9] Li M, Hao B, Zhang M, et al. Melatonin enhances radiofrequency-induced NK antitumor immunity, causing cancer metabolism reprogramming and inhibition of multiple pulmonary tumor development. Signal Transduct Target Ther. 2021;6(1):330. Published 2021 Sep 1. doi:10.1038/s41392-021-00745-7(IF:18.187)

[10] Qi S, Zhu Y, Liu X, et al. WWC proteins mediate LATS1/2 activation by Hippo kinases and imply a tumor suppression strategy. Mol Cell. 2022;82(10):1850-1864.e7. doi:10.1016/j.molcel.2022.03.027(IF:17.970)

[11] Zhu J, Li X, Cai X, et al. Arginine monomethylation by PRMT7 controls MAVS-mediated antiviral innate immunity. Mol Cell. 2021;81(15):3171-3186.e8. doi:10.1016/j.molcel.2021.06.004(IF:17.970)

[12] Teng KX, Niu LY, Xie N, Yang QZ. Supramolecular photodynamic agents for simultaneous oxidation of NADH and generation of superoxide radical. Nat Commun. 2022;13(1):6179. Published 2022 Oct 19. doi:10.1038/s41467-022-33924-3(IF:17.694)

[13] Zhong J, Guo Y, Lu S, et al. Rational design of a sensitivity-enhanced tracer for discovering efficient APC-Asef inhibitors. Nat Commun. 2022;13(1):4961. Published 2022 Aug 24. doi:10.1038/s41467-022-32612-6(IF:17.694)

[14] Liu F, Wang X, Duan J, et al. A Temporal PROTAC Cocktail-Mediated Sequential Degradation of AURKA Abrogates Acute Myeloid Leukemia Stem Cells. Adv Sci (Weinh). 2022;9(22):e2104823. doi:10.1002/advs.202104823(IF:16.806)

[15] Ji C, Qiu M, Ruan H, et al. Transcriptome Analysis Revealed the Symbiosis Niche of 3D Scaffolds to Accelerate Bone Defect Healing. Adv Sci (Weinh). 2022;9(8):e2105194. doi:10.1002/advs.202105194(IF:16.806)

[16] Feng L, Dou C, Xia Y, et al. Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery. ACS Nano. 2021;15(2):2263-2280. doi:10.1021/acsnano.0c07973(IF:15.881)

[17] Wang Z, Gong X, Li J, et al. Oxygen-Delivering Polyfluorocarbon Nanovehicles Improve Tumor Oxygenation and Potentiate Photodynamic-Mediated Antitumor Immunity. ACS Nano. 2021;15(3):5405-5419. doi:10.1021/acsnano.1c00033(IF:15.881)

[18] Jiang Z, He L, Yu X, et al. Antiangiogenesis Combined with Inhibition of the Hypoxia Pathway Facilitates Low-Dose, X-ray-Induced Photodynamic Therapy [published online ahead of print, 2021 Jun 25]. ACS Nano. 2021;10.1021/acsnano.1c01063. doi:10.1021/acsnano.1c01063(IF:15.881)

[19] Gong X, Li J, Xu X, et al. Microvesicle-inspired oxygen-delivering nanosystem potentiates radiotherapy-mediated modulation of tumor stroma and antitumor immunity. Biomaterials. 2022;290:121855. doi:10.1016/j.biomaterials.2022.121855(IF:15.304)

[20] Deng J, Xu W, Lei S, et al. Activated Natural Killer Cells-Dependent Dendritic Cells Recruitment and Maturation by Responsive Nanogels for Targeting Pancreatic Cancer Immunotherapy. Small. 2022;18(44):e2203114. doi:10.1002/smll.202203114(IF:15.153)