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All Answers2
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Protein9337
28 days ago
国产替代可以考虑，但别只问批间差，要拿同批次COA和验证数据：检测抗体配对、标准品溯源、板间和板内CV、回收率、种属交叉反应、稳定性。上线前重新做精密度是稳妥做法，尤其是细胞上清和血清基质。预算有限时可选96T或可拆板条，标准品分装冻存，避免反复冻融。优先选售后明确、能提供技术答疑和留样比对支持的供应商。
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KitBase8867
28 days ago
300份炎症因子筛查，我更建议先看样本基质再选平台。血清和细胞上清通常可先考虑ELISA，成本可控且适合多因子筛查；化学发光灵敏度可能更高，但受仪器、标准品、基质干扰影响。货期紧张时可找同靶点、同种属、同基质说明书的替代，但务必用少量旧样本做桥接：平行CV、稀释回收、标曲四参数拟合、低中高质量QC。不要直接用新试剂盒替换历史结论。
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